5-溴水杨醛氨基酸Schiff碱及其铜(Ⅱ)配合物的制备和抑菌性能研究

目的]研究Schiff碱抑菌活性的影响因素,为寻找理想的抗癌、抑菌药物提供参考。方法]合成5种5-溴水杨醛氨基酸Schiff碱配体,并分别与铜（II）形成配合物。采用红外光谱、紫外光谱进行表征,并分别进行抑菌试验。结果]从红外光谱数据及紫外光谱数据中可以看出,5-Br-Sal与AA完全反应生成了Schiff碱配体,该类配体与铜（II）形成配合物时,C-O中的氧原子以及C=N中的氮原子参与了对铜（II）的配位作用;同Sal-AASchiff碱相比,5-Br-Sal-AASchiff碱E2带、B带、R带均发生了红移,从紫外光谱图中可以看出,配合物的吸收峰位置相对于配体而言均发生了蓝移。从抑菌试验结果可以看出,5种配体中的抑菌圈大小顺序为5-Br-Sal-Gly〉5-Br-Sal-Ala〉5-Br-Sal-Met〉5-Br-Sal-Glu〉5-Br-Sal-Tyr,其中5-Br-Sal-Gly的抑菌圈直径最大。通过比较5-Br-Sal-AA及其与铜（II）形成配合物的抑菌圈直径可以看出,配合物的抑菌活性均高于相应配体的抑菌活性。结论]氨基酸R基团体积直接影响配体及配合物的抑菌活性,R基团体积越大,抑菌活性越弱。配体与铜（II）形成配合物后其抑菌活性增强。

Research on the Preparation and Antibacterial Activity of 5-Bromosalicylaldimine Amino Acid Schiff Alkali and Its Copper (Ⅱ) Compound

Objective] The reference for the medicine preparation of anti-cancer and anti-bacteria was provided through the study on the factors affecting the antibacterial activity of Schiff alkali.Method] Five types of 5-Bromosalicylaldimine amino acid(Glu、Met、Tyr、Gly and Ala) Schiff alkalis were produced and their complexes with copper(Ⅱ) were synthesized,which were experimented and characterized in antibacterial activity with infra-red spectrum and ultraviolet spectra.Results] The experimental result indicated that Schiff alkali complex could be formed through the complete reaction between 5-Br-Sal and AA,when which was combined wit the copper(Ⅱ) for the formation of the complex,the oxygen atom in C-O and nitrogen atom in C=N were paired with the copper(Ⅱ).Compared with Sal-AA Schiff alkali,band E2,band B and band R in 5-Br-Sal-AA Schiff alkali were moved to red zone and it can be seen from the UV spectrum that the position of the absorption peak of the complex was moved to blue zone.The experimental result in antibacterial test showed that the diameter of the inhibition zone of five types was 5-Br-Sal-Gly > 5-Br-Sal-Ala > 5-Br-Sal-Met > 5-Br-Sal-Glu > 5-Br-Sal-Tyr,among which,the largest diameter of the inhibition zone appeared in the type of 5-Br-Sal-Gly.The antibacterial activity of the complex was higher than the corresponding type form the comparison of the inhibition zone diameter.Conclusion] The size of the amino acid group R directly affected the antimicrobial activity of the type and its complex.The larger the size of group R was,the weaker the antimicrobial activity was.The antimicrobial activity of the complex from the type mixed with copper(Ⅱ) was increased.