| 药用植物灵菊七的染色体核型研究 |
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| 引用本文： | 范三微,董丽辉,凌庆枝,屠晓玮,林芝,高莉莉,魏兆军.药用植物灵菊七的染色体核型研究[J].安徽农业科学,2012(18):9644-9645,9650. |
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| 作者姓名： | 范三微 董丽辉 凌庆枝 屠晓玮 林芝 高莉莉 魏兆军 |
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| 作者单位： | 1. 浙江医药高等专科学校,浙江宁波,315100
2. 合肥工业大学生物与食品工程学院,安徽合肥,230009 |
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| 基金项目： | 浙江省中医药科技计划项目，浙江省大学生科技创新计划项目 |
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| 摘 要： | 目的]研究药用植物灵菊七(Gynura medica)的细胞染色体特点,并对其染色体核型进行分析。方法]以灵菊七幼苗茎尖为材料,从上午6:30～10:30,每隔20 min取材一次,采用常规制片技术制片,显微镜观察中期分裂相细胞,对染色体完全分散开的细胞进行染色体计数及核型分析。结果]在染色体分散良好的102个中期分裂相细胞中,有96.08%含20条染色体,3.92%含40条染色体;同时结果表明上午7:50～9:30取材较佳。结论]G.medica为二倍体植物,体细胞含有10对染色体,核型公式为2n=2x=20 m,染色体核型属于1A型。该研究结果为国内首次报道。
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| 关 键 词： | 灵菊七 染色体 核型 |
Study on Chromosomal Karyotypes of Medicinal Plant Gynura medica |
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| Institution: | FAN San-wei et al(Zhejiang Pharmaceutical College,Ningbo,Zhejiang 315100) |
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| Abstract: | Objective] To study the chromosomal characteristics and karyotypes of G.medica.Method] Stem tip of G.medica seedlings were collected every 20 min from 6:30 to 10:30 am,and all materials were treated and squashed for investigating chromosomal number and karyotypes analysis.Result] In total 102 stem tip cells in metaphase,96.08% of cells are with 20 chromosomes,and 3.92% of cells are with 40 chromosomes.The optimal time for sampling was from 7:50 to 9:30 am.Conclusion] G.medica is the diploid plant with 20 chromosomes.Karyotype analysis gets formula 2n = 2x = 20 m,and all 10 pairs of chromosomes belong to type 1A.The article reported the chromosomes characteristics of G.medica for the first time in China. |
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| Keywords: | Gynura medica Chromosome Karyotype |
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