| 转Bt基因抗虫棉的筛选和抗性鉴定 |
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| 引用本文： | 李长福,吴振廷,张玉翠,唐庆峰,林华峰.转Bt基因抗虫棉的筛选和抗性鉴定[J].安徽农业科学,2002,30(5):737-739. |
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| 作者姓名： | 李长福 吴振廷 张玉翠 唐庆峰 林华峰 |
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| 作者单位： | 1. 安徽农业大学植物保护系,合肥,230036;2. 郎溪县钟桥农技站 |
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| 摘 要： | 2000～2002年采用卡那霉素叶片涂抹法和室内饲喂生物测定法筛选和鉴定转Bt基因棉原始群系"20-1"2代,对单株进行了抗虫性鉴定和抗性级别评价.在供试的321株材料中,筛选出特高抗棉株22株,高抗棉株19株,并将这些棉株的自交铃种子种成株行,进行了纯合株系(抗性基因纯合)的筛选,筛选出特高抗8个株行,高抗10个株行,可进行进一步的转Bt基因棉的品种选育.
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| 关 键 词： | 转Bt基因棉 筛选 抗性鉴定 |
| 文章编号： | 0517-6611(2002)05-0737-03 |
Screen and Identification of Bt-transgenic Cotton Lines with the Resistance to Pest |
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| Li Changfu et al.Screen and Identification of Bt-transgenic Cotton Lines with the Resistance to Pest[J].Journal of Anhui Agricultural Sciences,2002,30(5):737-739. |
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| Authors: | Li Changfu |
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| Abstract: | The bioassay with larvae of bollworm were used to identify and screen the resistance of Bt-transgenic cotton original group "20-1"to insects.22 top-high resistant plants and 19 high resistant plants were screened among 321 original plants.The seeds of the same plant were planted in line.8 top-high resistant lines and 10 high resistant lines were screened. |
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| Keywords: | Bt-transgenic cotton Screen Identification of resistance to pest |
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