罗非鱼鱼皮酸溶性胶原蛋白的制备工艺研究简

目的]研究从罗非鱼鱼皮中用酸溶剂提取胶原蛋白的最佳条件.方法]以罗非鱼鱼皮为原料,利用酸处理提取胶原蛋白.以提取溶液、浓度、浸提时间、料液比为因素,通过测定其羟脯氨酸含量,计算不同提取条件下胶原蛋白的得率.再利用响应面法对提取条件进行优化,对拟合多项式回归模型进行分析.结果]由响应面优化得到罗非鱼鱼皮中胶原蛋白最佳酸提取工艺条件为：乙酸浓度0.53 mol/L,浸泡时间64.8h、料液比1∶25 g/ml,此条件下胶原蛋白提取量试验均值为244.86 mg/g.结论]研究可为罗非鱼鱼皮中胶原蛋白的提取利用提供参考.

Study on the Extraction of Acid-solubl College from Oreochromis nilofscus Skin

Objective ] To study the optimum conditions of extracting collagen using acid solution from the skin of Tilapia. Method ] The ex- tracting technology of collagen using acid solution from the skin of Tilapia Was studied in this paper, Choosing acid type, concentration of acid solution extraction time and solid-liquid ratio as single factor experiment factors, ：the extracting results were achieved by measuring the hydroxyproline content, the yield of collagen under different extraction conditions were calculated. The optimal extracting conditions were in- vestigated based on box-behnken design and response surface methodology. Result] By analyzing the fitted polynomial regression model, the optimum parameters of extracting collagen from the skin of Tilapia were as follows ： soaking Tilapia skin in acetic acid solution 0.53 mol/L with solid-liquid ratio 1：25 g/ml at 4 ℃ for 64.8 h, and the optimized extraction led to a collagen content of 244.86 mg/g. Conclusion] The study will provide reference for extraction and utilization of collagen from skin of Tilapia.