| 辽东桤木的组织培养和快繁技术研究 |
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| 引用本文： | 蔡丹,刘军,陈红,恭伟.辽东桤木的组织培养和快繁技术研究[J].安徽农业科学,2006,34(7):1309-1310,1323. |
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| 作者姓名： | 蔡丹 刘军 陈红 恭伟 |
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| 作者单位： | 四川农业大学林学园艺学院,四川雅安,625014;四川农业大学林学园艺学院,四川雅安,625014;四川农业大学林学园艺学院,四川雅安,625014;四川农业大学林学园艺学院,四川雅安,625014 |
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| 摘 要： | 选用辽东桤木实生无菌苗的带芽茎段进行组织培养。结果表明,用浓度75%的酒精和0.1%的升汞分别消毒种子5和20 min后,接入附加5 g/L葡萄糖的MS培养基,实生无菌苗成活率可达48%,且实生苗抽出真叶片数多,植株健壮;用WPM+6-BA(0.6、1.0)mg/L+NAA 0.1 mg/L+葡萄糖30 g/L作为增殖培养基,增殖倍数高达4.2;用1/2 WPM+IBA 1.0 mg/L+AC 500 mg/L+葡萄糖30 g/L作为生根培养基,生根率达91%;通过逐步炼苗法炼苗,炼苗成活率达93%。
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| 关 键 词： | 辽东桤木 组织培养 快繁技术 |
| 文章编号： | 0517-6611（2006）07-1309-02 |
| 收稿时间： | 2005-11-02 |
| 修稿时间： | 2005-11-02 |
Study on Tissue Culture and Rapid Propagation Techniques of Alnus sibirica |
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| CAI Dan et al.Study on Tissue Culture and Rapid Propagation Techniques of Alnus sibirica[J].Journal of Anhui Agricultural Sciences,2006,34(7):1309-1310,1323. |
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| Authors: | CAI Dan |
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| Institution: | College of Forestry and Hortieuhural , Sichuan Agricultural University, Yaan, Siehuan 625014 |
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| Abstract: | The germfree cauline segments with axillary buds of Alnus sibirica was used as explants in tissue culture.The study showed that the best medium for the germination was MS dextrose 5 g/L and the survival rate of seedling could reach 48%;for multiplication,WPM 6-BA(0.6 or 1.0)mg/L NAA 0.1 mg/L dextrose 30 g/L and the rate of multiplication could reach 4.2 times and for rooting culture,1/2WPM IBA 1.0 mg/L AC 500 mg/L dextrose 30 g/L and the rate of rhizogenesis could reach 91%.After the plantlets being trained indoor for 23 d and then 37 d outdoor,the survival rate of transplanting could reach 93%. |
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| Keywords: | Alnus sibirica Tissue culture Rapid propagation technique |
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