| 紫花含笑的组织培养技术研究 |
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| 引用本文： | 朱碧华,黄宝祥,黄文超,符树根.紫花含笑的组织培养技术研究[J].安徽农业科学,2009,37(29):14024-14027. |
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| 作者姓名： | 朱碧华 黄宝祥 黄文超 符树根 |
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| 作者单位： | 江西科技师范学院,江西南昌,330013;江西省林业科学院,江西南昌,330032 |
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| 基金项目： | 江西省科技支撑计划项目 |
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| 摘 要： | ［目的］为紫花含笑的开发和大量繁殖提供参考。［方法］以紫花含笑成年树带芽茎段为材料，预处理后接种到诱导培养基上诱导不定芽，然后分别对不定芽进行增殖和生根培养，研究不同外植体处理方法、培养基、激素种类及配比对紫花含笑培养效果的影响。［结果］4 ℃低温预处理5～7 d可降低外植体的褐化及污染率；紫花含笑的最佳基本培养基为MS培养基，激素配比为6 BA 0.5 mol/L＋IBA 0.1 mol/L，继代周期以25 d为宜，增殖系数可达3.5以上；不定芽在培养基1/2 MS＋NAA 3 mg/L＋6 BA 0.1 mg/L上暗培养15 d，然后转入不加任何激素的1/2 MS培养基中，15 d即可出根，生根率为33%；生根苗栽于混合基质中，成活率可达85%。［结论］该研究建立了紫花含笑的组织培养技术。
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| 关 键 词： | 紫花含笑 培养基 激素 |
Study on Tissue Culture Technique for Michelia crassipes Law |
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| ZHU Bi-hua et al.Study on Tissue Culture Technique for Michelia crassipes Law[J].Journal of Anhui Agricultural Sciences,2009,37(29):14024-14027. |
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| Authors: | ZHU Bi-hua |
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| Institution: | ZHU Bi-hua et al (Jiangxi Normal University of Science , Technology,Nanchang,Jiangxi 330013) |
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| Abstract: | Objective]The aim was to provide the reference for development and large scale propagation of Michelia crassipes Law.Method] With the stems with tips from adult M.crassipes tree as the materials,they were inoculated into the inducing medium for adventitious bud induction after pretreatment,and then the adventitious buds were taken for multiplication and rooting culture.The effects of different treatment methods of transplants,mediums,hormone types and ratio on culture effect of M.crassipes were studied.R... |
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| Keywords: | Michelia crassipes Medium Hormone |
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