| 南极适冷菌Rheinheimera sp.97产抗菌活性物质发酵条件的优化 |
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| 引用本文： | 李贺,林学政,刘同军.南极适冷菌Rheinheimera sp.97产抗菌活性物质发酵条件的优化[J].安徽农业科学,2011,39(17):10101-10103,10118. |
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| 作者姓名： | 李贺 林学政 刘同军 |
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| 作者单位： | 国家海洋局第一海洋研究所,国家海洋局海洋生物活性物质重点实验室,山东,青岛,266061;山东轻工业学院食品与生物工程学院,山东,济南,250353;国家海洋局第一海洋研究所,国家海洋局海洋生物活性物质重点实验室,山东,青岛,266061;山东轻工业学院食品与生物工程学院,山东,济南,250353 |
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| 基金项目： | 海洋公益性行业科研专项(201005032-2); 国家863计划项目(2007AA091905) |
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| 摘 要： | 目的]优化南极适冷菌Rheinheimerasp．97产抗茵活性物质的发酵条件。方法]通过单因素和正交试验对南极适冷茵R．sp．97产抗茵活性物质的发酵培养基进行了优化，并通过单因素试验对其发酵条件进行了优化。结果]菌株R．sp．97产抗菌活性物质的最佳培养基为：蛋白胨3．Og／L，硫酸铵1．0g／L，淀粉2．0g／L，NaCl15．0g／L；最佳发酵条件为：发酵培养基初始pH8，发酵温度10℃，摇瓶装液量30．0％（V／V），接种量1．0％（V／V）。优化后菌株R．sp．97发酵液的抗茵活性较优化前提高了22．2％。结论]为研究南极适冷茵R．sp．9r7的活性物质奠定了基础。
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| 关 键 词： | 南极细菌 抗菌活性 发酵条件 优化 |
Optimization of Fermentation Medium and Conditions of Antibiotic Active Substances Produced by Antarctic Psychrotrophic Bacterium Rheinheimera sp. 97 |
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| LI He et al.Optimization of Fermentation Medium and Conditions of Antibiotic Active Substances Produced by Antarctic Psychrotrophic Bacterium Rheinheimera sp. 97[J].Journal of Anhui Agricultural Sciences,2011,39(17):10101-10103,10118. |
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| Authors: | LI He |
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| Institution: | LI He et al (Key Lab of Marine Bioactive Substances,SOA,First Institute of Oceanography,Qingdao,Shandong 266061) |
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| Abstract: | Objective] The aim was to optimize the fermentation conditions of antibiotic active substances produced by Antarctic psychrotrophic bacterium Rheinheimera sp. 97. Method] Single-factor experiment and orthogonal test were adopted to optimize the fermentation medium and conditions of antibiotic active substances produced by Antarctic psychrotrophic bacterium R. sp. 97, while the fermentation conditions were optimized by single-factor experiment. Result] The optimum fermentation medium for the antibiotic ac... |
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| Keywords: | Antarctic bacteria Antibacterial activity Fermentation conditions Optimization |
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