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蚕豆根尖细胞微核技术监测实验室有机废水的污染研究
引用本文:闫晶晶,屈艾,刘小燕,贾蓉.蚕豆根尖细胞微核技术监测实验室有机废水的污染研究[J].安徽农业科学,2011,39(17):10417-10419,10517.
作者姓名:闫晶晶  屈艾  刘小燕  贾蓉
作者单位:徐州师范大学生命科学学院,江苏,徐州,221116
摘    要:目的]对生物及化学类实验室的有机废水进行遗传毒性和污染效应的研究。方法]将实验室条件下制备环己酮时所排有机废水逐级稀释成8个梯度浓度,采用蚕豆(Viciafaba)根尖细胞微核技术进行检测。结果]不同浓度梯度的实验室有机废水对蚕豆根尖细胞有遗传毒性,在一定浓度范围内,随着废水处理浓度增大,蚕豆根尖细胞微核率、污染指数和有丝分裂指数逐渐增加;但浓度过高,反而会抑制蚕豆根尖细胞微核的形成和有丝分裂的进行。结论]实验室有机废水有明显的遗传毒性和环境污染效应,可以利用蚕豆根尖细胞微核技术对其污染效应进行监测和评价,实验室废水必须进行合理的处理(稀释或吸附消除重金属)方能排出。

关 键 词:实验室有机废水  微核技术  遗传毒性  污染效应

Study on Organic Wastewater Monitoring of Laboratory Using MCN Test of Vicia Faba Root Tips
YAN Jing-jing et al.Study on Organic Wastewater Monitoring of Laboratory Using MCN Test of Vicia Faba Root Tips[J].Journal of Anhui Agricultural Sciences,2011,39(17):10417-10419,10517.
Authors:YAN Jing-jing
Institution:YAN Jing-jing et al (School of Life Sciences,Xuzhou Normal University,Xuzhou,Jiangsu 221116)
Abstract:Objective] The genetic toxicity and pollution effects of biological and chemical organic wastewater were studied. Method] Organic wastewater which was formed during the preparation of cyclohexanone were diluted eight concentration gradients and detected using MCN test of Vicia faba root tips. Results] The results showed that different concentration gradients of organic wastewater had genetic toxicity. In certain concentration range, with the rising of organic wastewater treatment concentration, rate of m...
Keywords:Laboratory organic wastewater  Micronucleus technology  Genetic toxicity  Pollution effect  
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