| 黄粉虫酪氨酸羟化酶基因的克隆与序列分析 |
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| 引用本文： | 刘晶晶,韩宏岩,许维岸.黄粉虫酪氨酸羟化酶基因的克隆与序列分析[J].安徽农业科学,2011,39(17):10133-10134. |
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| 作者姓名： | 刘晶晶 韩宏岩 许维岸 |
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| 作者单位： | 苏州大学基础医学与生命科学学院,江苏,苏州,215123;苏州大学基础医学与生命科学学院,江苏,苏州,215123;苏州大学基础医学与生命科学学院,江苏,苏州,215123 |
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| 摘 要： | 目的]克隆鞘翅目昆虫黄粉虫酪氨酸羟化酶（TmTH）基因。方法]采用eDNA末端快速扩增（RACE）技术和PCR方法克隆了鞘翅目昆虫黄粉虫‰掰基因，并对其进行了生物信息学分析。结果]‰ⅢcDNA全长2103bp，包含1605bp的开放式阅读框，可编码534个氨基酸残基，蛋白质分子量为60.66kD，等电点为5．70，构建了其系统进化树。结论]m掰基因的克隆为鞘翅目昆虫酪氨酸’羟化酶的研究提供了参考。
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| 关 键 词： | 黄粉虫 酪氨酸羟化酶 基因克隆 系统进化树 |
Molecular Cloning and Sequences Analysis of the Tyrosine Hydroxylase Gene in Mealworm, Tenebrio molitor |
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| LIU Jing-jing et al.Molecular Cloning and Sequences Analysis of the Tyrosine Hydroxylase Gene in Mealworm, Tenebrio molitor[J].Journal of Anhui Agricultural Sciences,2011,39(17):10133-10134. |
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| Authors: | LIU Jing-jing |
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| Institution: | LIU Jing-jing et al (College of Preclinical Medicine and Biological Science,Soochow University,Suzhou,Jiangsu 215123) |
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| Abstract: | Objective] The research aimed to clone tyrosine hydroxylase gene of Tenebrio molitor (TmTH) of Coleoptera insects. Method] RACE and PCR methods of cDNA tail end was used to clone TmTH gene of Coleoptera insects, and bioinformatics analysis was carried out. Result] The overall length of TmTH cDNA was 2 103 bp, which including 1 605 bp open reading frame (ORF), thus can encoding a protein of 534 amino acid residues, and the molecular weight and pI of TmTH were 60.66 kD and 5.70,respectively. Based on that,... |
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| Keywords: | Tenebrio molitor Tyrosine hydroxylase Gene cloning Phylogenetic tree |
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