| 高简并引物小温度范围不规则降落PCR在庞大基因家族扩增中的应用 |
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| 引用本文： | 华慧颖,王芳,常重杰,杜启艳.高简并引物小温度范围不规则降落PCR在庞大基因家族扩增中的应用[J].安徽农业科学,2011(17):10164-10166. |
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| 作者姓名： | 华慧颖 王芳 常重杰 杜启艳 |
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| 作者单位： | 中州大学化工食品学院;河南师范大学生命科学学院; |
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| 摘 要： | 目的]探讨高简并性引物扩增庞大基因家族基因的特殊方法。方法]采用高简并引物对鲤鱼基因组DNA分别进行了常规PCR扩增、常规降落PCR扩增以及优化后的小温度范围不规则跳跃降落PCR扩增。结果]采用常规PCR仅得1条明显条带，得到的基因较少；采用常规降落PCR仅得到弥散性扩增，无明显条带出现；而采用小温度范围不规则跳跃降落PCR则得到了3条明显条带和多个基因，扩增结果理想。结论]为庞大基因家族扩增条件的优化与选择提供了理论依据。
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| 关 键 词： | 高简并性引物 不规则降落PCR 基因家族 扩增 |
Application of Abnormal Touchdown PCR with High Degeneracy Primer in Amplification of Large-family Genes |
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| HUA Hui-ying et al.Application of Abnormal Touchdown PCR with High Degeneracy Primer in Amplification of Large-family Genes[J].Journal of Anhui Agricultural Sciences,2011(17):10164-10166. |
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| Authors: | HUA Hui-ying |
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| Institution: | HUA Hui-ying et al (College of Chemical Engineering and Food,Zhongzhou University,Zhengzhou,Henan 450044) |
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| Abstract: | Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy. Method] By using the primers with high degeneracy, conventional PCR, conventional touchdown PCR and the optimized abnormal touchdown PCR were respectively carried out to amplify the genomic DNA of Cyprinus carpio. Result] Only one evident electrophoretic band and a few Sox genes were obtained by using normal PCR; no obvious electrophoretic band but dispersive product was ob... |
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| Keywords: | Primers with high degeneracy Abnormal touchdown PCR Large-family genes Amplification |
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