| 一株大凉疣螈肠道弗氏柠檬酸杆菌的分离及其药敏试验 |
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| 引用本文： | 李蓓,邹立扣,罗燕.一株大凉疣螈肠道弗氏柠檬酸杆菌的分离及其药敏试验[J].安徽农业科学,2011,39(18):11078-11080. |
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| 作者姓名： | 李蓓 邹立扣 罗燕 |
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| 作者单位： | 四川农业大学都江堰校区微生物学实验室,四川,都江堰,611830 |
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| 基金项目： | WWF中国物种项目野生动植物保护小额基金项目(CN086-1.01-SM01) |
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| 摘 要： | 首次从大凉疣螈（Tylototriton taliangensis）肠道分离获得弗氏柠檬酸杆菌（Citrobacter freundii），利用分子生物学方法对其16SrD—NA进行了扩增测序，并与GenBank中已有的有关序列进行比较，进行了系统发育分析．．测序结果表明，16SrDNA与GenBank中序列同源率在98％～99％，分离的菌株与GUl26681（C．freundii MRB0903）处于同一分支中；序列同源性及系统发育树表明，此菌为弗氏柠檬酸杆菌；药敏试验表明，弗氏柠檬酸杆菌对氨苄西林及头孢噻吩中介，对其余药物均敏感。
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| 关 键 词： | 大凉疣螈 肠道 弗氏柠檬酸杆菌 药敏试验 |
Isolation and Susceptibility Test of an Intestinal Citrobacter freundii from Tylototriton taliangensis |
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| LI Bei et al.Isolation and Susceptibility Test of an Intestinal Citrobacter freundii from Tylototriton taliangensis[J].Journal of Anhui Agricultural Sciences,2011,39(18):11078-11080. |
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| Authors: | LI Bei |
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| Institution: | LI Bei et al(Lab.of Microbiology,Dujiangyan Campus of Sichuan Agricultural University,Dujiangyan,Sichuan 611830) |
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| Abstract: | A Citrobacter freundii was isolated from the intestinal tact of Tylototriton taliangensis,and its 16S rDNA was amplified and sequenced by the molecular biology method,and then the 16S rDNA was compared with other sequences in Genbank to conduct a phylogenetic analysis.The results indicated that the 16S rDNA sequence of Citrobacter freundii showed 98%-99% homogeny with those in the GenBank,and belonged to the same branch with GU126681(C.freundii MRB0903).As shown by the sequence homology and phylogenetic tre... |
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| Keywords: | Tylototriton taliangensis Intestinal tract Citrobacter freundii Antimicrobial susceptibility test |
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