| 白假丝酵母脂肪酶CaLIP5在毕赤酵母X33中的优化重组表达 |
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| 引用本文： | 杨宁,蓝东明,王永华,杨博.白假丝酵母脂肪酶CaLIP5在毕赤酵母X33中的优化重组表达[J].安徽农业科学,2011(17):10124-10126,10129. |
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| 作者姓名： | 杨宁 蓝东明 王永华 杨博 |
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| 作者单位： | 华南理工大学生物科学与工程学院;华南理工大学食品科学与工程学院; |
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| 基金项目： | 广州市科技攻关项目(2009A1-E) |
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| 摘 要： | 目的]研究新的脂肪酶及优化脂肪酶发酵培养条件。方法]在毕赤酵母X33系统中重组表达白假丝酵母脂肪酶CaLIP5。利用响应面分析法，构建了CaLIP5的酶活回归方程。结果]由响应面分析可知，在温度25．71oC，pH8．33，酵母提取物浓度1．28％和葡萄糖浓度4．06％时，预测最大酶活为8．33U／ml。试验验证酶活为9．24U／ml。实际试验值与模型预测值基本一致。优化后的酶活提高了22．22％。结论]在优化的培养条件下，可以获得毕赤酵母X33重组表达CaLIP5的最大酶活。
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| 关 键 词： | 白假丝酵母脂肪酶 重组表达 优化培养基 响应面法 |
Optimum Production of Recombinant Candida albicans Lipase 5 Expressed in Pichia pastoris X33 |
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| YANG Ning et al.Optimum Production of Recombinant Candida albicans Lipase 5 Expressed in Pichia pastoris X33[J].Journal of Anhui Agricultural Sciences,2011(17):10124-10126,10129. |
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| Authors: | YANG Ning |
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| Institution: | YANG Ning et al (School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006) |
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| Abstract: | Objective] To develop new lipases and optimize the fermentation medium of lipase. Method] The lip5 gene of C. albicans was first cloned and heterogenously expressed in Pichia pastoris X33 system. Response surface methodology was adopted to construct the regression equation for the lipolytic activity of CaLIP5. Result] Under 25.71 ℃, pH 8.33, 1.28% yeast extract, and 4.06% glucose concentration, the predicted maximum lipolytic activity was 8.33 U/ml, and the actual value was 9.24 U/ml. The actual value and the predicted value were basically the same. The optimized lipolytic activity was 22.22% higher than before. Conclusion] Highest lipolytic activity of recombinant CaLIP5 was obtained in the optimized fermentation progress. |
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| Keywords: | Lipase of Candida albicans Heterogeneous expression Optimization of medium Response surface methodology |
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