利用根尖染色体鉴别药用植物丹参、甘西鼠尾的种子

目的]鉴别丹参和甘西鼠尾的种子。方法]种子25℃萌发,待根尖长0.5~1.0cm,冰箱4℃冷藏预处理24h,卡诺固定液固定1~1.5h,1∶1的浓盐酸和95%乙醇解离10~15min,改良的石碳酸品红液染色,镜检取分散良好的标本,冰冻揭盖片,中性树胶封片显微照像、计数、测量。结果]两者在染色体的形态和结构上有明显差异。结论]利用根尖染色体可以有效鉴别丹参和甘西鼠尾的种子。

Identification of Salvia miltiorrhiza Bge.and Salvia przewalskii Maxim Seed through Chromosome in Root Tip

Objective] The seeds of Salvia militorrhiza Bge.and Salvia przewalskii Maxim were identified.Method] Seeds were sprouted at 25 ℃.The root-tip with 0.5～1.0 cm length were cut and treated in 4 ℃ refrigerator for 24 hours,fixed for 1～1.5 hours with Carnoy’s fixative,differentiated for 10-15 min with hydrochloric acid and Ethanol 95 %(1∶1),pressed to section in carmine for staining. Them were observed under microscope,then the samples that differentiated well chosen,covered on frozen station with mountin Canada balsam(neutral mounting medium),for micro-photographing,counting and measuring.Result]There were significant differences in the morphology and the structure.Conclusion] Salvia militorrhiza Bge.and Salvia przewalskii Maxim seed can be identified effectively through chromosomal technique.