| 山药叶片总RNA提取方法的比较 |
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| 引用本文： | 张晓娟,胡选萍.山药叶片总RNA提取方法的比较[J].安徽农业科学,2010,38(33):18728-18729. |
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| 作者姓名： | 张晓娟 胡选萍 |
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| 作者单位： | 陕西理工学院生物科学与工程学院,陕西汉中,723001;陕西理工学院生物科学与工程学院,陕西汉中,723001 |
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| 基金项目： | 陕西理工学院引进人才科研启动项目，陕西省教育厅专项科研项目 |
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| 摘 要： | 目的]提取高质量的山药叶片总RNA。方法]以山药叶片为材料,采用异硫氰酸胍法、改良CTAB法提取山药叶片总RNA并进行比较分析,利用琼脂糖凝胶电泳分析所提RNA的完整性,并用紫外分光光度计分析RNA的纯度。结果]改良CTAB法提取的总RNA的28S、18SrRNA条带清晰、整齐,测得A260/A280为1.72.0。结论]改良的CTAB法适合山药叶片总RNA提取。
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| 关 键 词： | 山药叶片 总RNA提取 改良CTAB法 异硫氢酸胍法 |
Comparative Study on Extraction Method for Total RNA from Leaves of Dioscorea opposita |
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| Institution: | ZHANG Xiao-juan et al ( School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001 ) |
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| Abstract: | Objective]The aim was to obtain high-quality total RNA from leaves of Dioscorea opposita.Method]The guanidinium isothiocyanate method and modified CTAB method was used to extract total RNA from leaves of Dioscorea opposita respectively.By employing agarose gel electrophoresis and UV-spectrophotometer,the integrity and purity of total RNA were analyzed.Result]The 28S and 18S bands of total RNA extracted by the modified CTAB method were clear and visible,and the A260/A280 value of it was from 1.7 to 2.0.Conclusion]Modified CTAB method was suitable for the extraction of total RNA from Dioscorea opposita leaves. |
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| Keywords: | Dioscorea opposita leaves Extraction of total RNA Modified CTAB method Guanidinium isothiocyanate method |
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