孔雀石绿在水产品中残留检测的研究

目的]为了对孔雀石绿及其代谢产物隐色孔雀石绿的残留研究有更全面、更深入的了解,并为进一步开展孔雀石绿残留检测工作提供参考。方法]从化学检测法和生物检测法2个方面分别综述了孔雀石绿在水产品中残留检测的主要方法,包括薄层色谱法、分光光度法、共振瑞利散射法、气质联用法、高效液相色谱法和酶联免疫法等。结果]由于MG和LMG的活性很强,其前处理过程复杂,有待进一步简化并提高回收率。同时,MG和LMG的最大吸收波长1个在可见光范围,1个在紫外范围。给其同时测定带来了一定的难度,因而其残留分析过程比较复杂。结论]基层的大规模筛选检测以免疫学检测方法更适用更有应用前景,但国际公认的确证方法仍是化学分析法。

Study on Residue Determination of Malachite Green in Aquatic Products

The aim of this experiment is mainly to give a comprehensive overview of residue determination of malachite green(MG) and its metabolites leuco malachite green(LMG) in aquatic products from two aspects of chemical and biological detection method respectively and offer us references for future malachite green residue determination.The systems for malachite green residues determination are discussed in thin-layer chromatography,spectrophotometry,resonance Rayleigh scattering method,GC-MS HPLC and ELISA method.The results showed that MG and LMG have very strong activity and pre-treatment process needs further simplification and recovery improvement.Meanwhile,one of the maximum absorption wave length of MG and LMG lies in the visible range while another maximum absorption wave length of MG and LMG lies in the ultraviolet range.This has provided certain difficulties in simultaneous determination.Therefore,their residue analysis process remains fairly complicated.In the grass-root institutions,it has more application prospect to undertake immunity detection for large-scale screening detection;however,the universally agreed confirmatory method is still chemical detection method.