酸枣根中鞣质含量测定及提取工艺研究

目的]优化酸枣根鞣质的提取工艺,建立稳定的酸枣根中鞣质的含量测定方法。方法]用干酪素法测定总鞣质的含量,并以提取物中鞣质含量为评价指标,以丙酮浓度、提取时间、料液比及提取次数为考察因素,采用正交设计法筛选酸枣根的提取工艺条件。结果]提取次数为主要影响因素;最佳提取条件为:超声提取的丙酮浓度为70%,料液比为1∶20(W/V,g/ml,下同),提取时间为30 min,提取3次;在此条件下,酸枣根中鞣质的含量为6.82%。结论]所优选的鞣质提取工艺合理,操作简便可行。

Determination of the Tannins in Ziziphus jujuba Roots and Its Extraction Technology

Objective] To optimize the extraction technology of tannins from Ziziphus jujuba roots,and to establish stable determination method of tannins content in Z.jujuba roots.Method] Content of total tannins was detected by casein method.With tannins content as the evaluation index,the extraction time,solid-liquid ratio,extraction times,and acetone concentration were taken as the investigation factors.Extraction technology conditions were optimized by orthogonal design.Result] Results showed that extraction times was the major influencing factor.The optimal extraction condition was as follows: 70% acetone concentration,1∶20(W/V,g/ml)solid-liquid ratio,30 min extraction time and 3 extraction times.Under this condition,the tannins content in Z.jujuba roots reached 6.82%.Conclusion] The optimized extraction technology was reasonable,simple and feasible.