不同发育类型的麦冬块根中黄酮含量变化规律比较

目的]研究国产优质麦冬块根不同发育阶段的麦冬黄酮含量的相关性规律,确定其品质特征,为麦冬GAP质量控制及资源综合开发利用提供科学依据。方法]在杭麦冬(3年生)和川麦冬(1年生)主产区采集植株块根样品,按照鲜块根等级划分标准分级,微波杀酶-50℃恒温烘干,采用紫外分光光度法分别测定各等级麦冬块根的黄酮含量,并运用SPSS17.0版统计软件分析不同发育阶段麦冬块根平均生物量与麦冬黄酮含量的相关性规律。结果]不同发育阶段的麦冬块根的黄酮平均含量分别为杭麦冬0.121%～0.192%和川麦冬0.135%～0.208%;随着麦冬植株块根发育阶段进程增加,麦冬块根的内含物积累总量与麦冬黄酮含量呈极显著的负相关降低(P<0.001),其回归方程分别为:杭麦冬y1=-0.047x+0.2(r2=0.997);川麦冬y2=-0.050x+0.217(r2=0.992)。结论]麦冬块根阶段发育的内含物积累总量与麦冬次生代谢产物黄酮的含量密切相关,国产麦冬块根的传统等级规格的划分是有一定科学道理的。

A Preliminary Study of the Variation of the Flavonoids Content of Domestic High Quality Ophiopogon japonicus (Thunb) Ker-Gawl.Tubers at Different Development Stages

Objective] To study relevance rules of flavonoids content of domestic high-quality Ophiopogon japonicus(Thunb) Ker-Gawl.tubers at different developmental stages and determine their quality characteristics,so as to provide scientific basis for GAP quality control and utilization of O.japonicus.Methods] O.japonicus tubers from Hangzhou City(2-year-old) and Sichuan Province(1-year-old) were graded in accordance with the fresh tuber grading standards.After killing enzyme in microwave and drying at-50 ℃,the flavonoids content of O.japonicus tubers was determined by ultraviolet spectrophotometry.SPSS17.0 version statistical software was adopted to analyze the law of correlation between the average biomass and the flavonoids content of tubers at different developmental stages.Results] The average flavonoids content of Hangzhou and Sichuan O.japonicus tubers at different developmental stages was 0.121%-0.192% and 0.135%-0.208%,respectively.With the increase of developmental stage,the accumulation of photosynthetic products was negatively related to the flavonoids content of O.japonicus tubers(P<0.001).The regression equation was written as y1=-0.047x+0.2(R2=0.997) for Hangzhou O.japonicus and y2=-0.050x+0.217(R2=0.992) for Sichuan O.japonicus.Conclusion] The accumulation of photosynthetic products in O.japonicus tubers at different developmental stages is closely related to the content of secondary metabolites flavonoids.It is scientific to divide the domestic high quality O.japonicus tubers by the traditional grade specifications.