宁夏常用玉米自交系的SSR遗传分析 |
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引用本文: | 李新,佘奎军,罗湘宁,许志斌.宁夏常用玉米自交系的SSR遗传分析[J].安徽农业科学,2011,39(35):21594-21596. |
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作者姓名: | 李新 佘奎军 罗湘宁 许志斌 |
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作者单位: | 宁夏农林科学院农作物研究所,宁夏永宁,750105 |
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基金项目: | 宁夏自治区自然科学基金项目 |
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摘 要: | 目的]对宁夏常用的82份玉米(Zea may L.)自交系进行遗传分析,划分自交系类群,以提高玉米育种效率。方法]利用SSR标记中可用于遗传多样性分析的20对SSR引物对材料进行分析。结果]82份自交系中共检测出335个等位基因,其中具有多态性的为108个,每对引物检测出3~10个多态性位点,平均为5.4个。SSR引物的多态性信息量(PIC)介于0.523 3~0.858 9,平均为0.7123。结论]82份自交系划分为5个类群,分类结果基本与系谱来源一致。
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关 键 词: | 玉米自交系 SSR 遗传分析 |
Study of Genetic Diversity in Maize(Zea may L.) Inbred Lines of Ningxia by SSR Markers |
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Institution: | LI Xin et al(Crop Institution of Ningxia Academy of Agricultural and Forestry Science,Yongning,Ningxia 750105) |
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Abstract: | Objective] In order to classify maize inbred genetic group and increase the breeding efficiency,the genetic variation of 82 maize inbred lines in Ningxia was analyzed.Method] One types of molecular marker,simple sequence repeats(SSR) was assayed to determine the genetic diversity of these materials.Result] 20 core-primers could test 335 allele sites in sample of 82 inbred lines,among them 108 were polymorphic.The average number of allele per SSR locus was 5.4 with arranging from 3 to 10.The average of polymorphic information content(PIC) was 0.712 3 with arranging from 0.523 3 to 0.858 9.Conclusion] The inbred lines are classified into 5 groups and the results agree with the pedigree of the inbre line. |
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Keywords: | Maize inbred line SSR Genetic diversity |
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