金银花中绿原酸的提取和含量测定

目的]研究金银花中绿原酸的不同提取方法并测定了不同花期金银花中绿原酸的含量。方法]以金银花为材料,采用乙醇回流法和超声提取法提取金银花中的绿原酸,并以紫外分光光度法测定提取物中绿原酸的含量,探讨了不同开花时期金银花中绿原酸含量的差异。结果]以乙醇回流法提取的金银花原液中绿原酸浓度为1.905 mg/ml,含量为3.81%,而以超声提取法提取的金银花原液中绿原酸浓度为1.306 mg/ml,含量为2.61%,故乙醇回流法比超声提取法提取率更高一些;以乙醇回流法提取的全开金银花原液中绿原酸浓度为0.167 7 mg/ml,含量为0.34%,而以乙醇回流法提取的花苞原液中绿原酸浓度为0.260 1 mg/ml,含量为0.52%,故花苞中的绿原酸含量要高一些。结论]该方法操作简便迅捷,可用于金银花中绿原酸的提取和含量测定。

Extraction and Quantitative Determination of Chlorogenic Acid from FLOS LONICERAE JAPONICAE

Objective]To determine the content of chlorogenic acid in extracts of FLOS LONICERAE JAPONICAE at different flowering periods that were prepared by different method.Method]The chlorogenic acid(CA) in FLOS LONICERAE JAPONICAE was determined and identified by UV spectrophotometric method after extraction with ethanol reflux method or ultrasonic method,aiming at discussing the difference of chlorogenic acid at different flowering periods.Result]The concentration and content of chlorogenic acid in original extract of FLOS LONICERAE JAPONICAE prepared by ethanol reflux method were 1.905 mg/ml and 3.81%,while those in original extract prepared by supersonic method were 1.306 mg/ml and 2.61%.Compared with supersonic method,ethanol reflux method was optimized and validated.Moreover,the concentration and content of chlorogenic acid in original extract of L.japonica flower prepared by ethanol reflux were 0.167 7 mg/ml and 0.34%,while those in original extract of L.japonica bud were 0.260 1 mg/ml and 0.52%.Hence,the bud contained more chlorogenic acid.Conclusion]The method is easy,simple and rapid to extract chlorogenic acid and determine its content in FLOS LONICERAE JAPONICAE.