湘西产槲蕨不同种质资源间总黄酮含量差异研究

目的]研究湘西产槲蕨不同种质资源间总黄酮含量的差异。方法]用微波法提取槲蕨中的总黄酮和总柚皮苷,以芦丁为标准品,用比色法测定8份槲蕨种质资源叶、叶柄和根状茎的总黄酮含量;另以柚皮苷为标准品,比色法测定槲蕨不同部位的总柚皮苷含量。结果]湘西产槲蕨种质资源间总黄酮含量的差异较大。龙山样品的总黄酮含量最高,为13.5%;王村样品的次之;保靖样品的最低,仅有1.8%;槲蕨不同部位中柚皮苷含量高低的分布规律为叶〉根状茎〉叶柄。结论]龙山样品的总黄酮含量最高,可作为人工栽培时的原种利用;而叶中总柚皮苷含量最高,可对槲蕨的叶加以利用和开发,为槲蕨的种质资源筛选和人工栽培提供试验依据。

Differences of Total Flavone Content in Xiangxi Drynaria fortunei among Different Germplasm Resources

Objective]To study the differences of total flavone content in Xiangxi Drynaria fortunei among different germplasm resources.Method]The total flavone and total naringin were extracted from Drynaria fortunei by microwave method.With rutin as standard substance,the content of total flavone in leaf,petiole and rhizome of 8 Drynaria fortunei germplasm resources harvested in Xiangxi was determined by colorimetry.In addition,with naringin as standard substance,the content of total naringin in different parts of Drynaria fortunei was determined by colorimetry.Result]There was significant difference about the content of total flavone in Xiangxi Drynaria fortunei among different germplasm resources.More specifically,the content of total flavone in Longshan samples was the highest at 13.5%,followed by Wangcun samples at 1.8%,and the least in Baojing samples.Moreover,the content of total naringins in different parts of Drynaria fortunei followed a trend of leaf > rhizome > petiole.Conclusion]Drynaria fortunei harvested from Longshan County could be used as the original species for artificial cultivation because of the highest content of total flavone.Besides,Drynaria fortunei leaves could be utilized and developed for the highest content of total naringin,aiming at providing experimental foundation for the research on germplasm resources screen and artificial cultivation of Drynaria fortunei.