小麦品种SARKA抗白粉病基因遗传分析及SSR标记初选

目的]对小麦品种SARKA的抗白粉病基因进行遗传分析。方法]SARKA（抗）×河农822（感）的F1代表现为感病，对其F2代分离群体300株进行抗病、感病鉴定；利用χ^2测验进行统计分析；采用集团分离分析法（BSA）对F2代群体建立抗病DNA池和感病DNA池，选取均匀分布于小麦21个连锁群上的165对引物对抗感DNA池以及双亲进行SSR引物筛选。结果]经过χ^2测验符合1：3一对等位基因的分离规律，抗性分析表明，SARKA的抗病性是由一对隐性基因控制。结论]位于1A染色体上的SSR标记Xgwm99、Xgwm357与SARKA的抗白粉病基因连锁程度较高，遗传距离分别为34．7和29．9cM。

Research on the Genetic Analysis of the Wheat Variety SARKA Gene Resisting Powdery Mildew and SSR Marker Selection

Objective]The genetic analysis of wheat variety SARKA gene resisting the powdery mildew was done.Method]F_1 from SARKA (anti)×Henong 822(sensitive) was sensitive to the powdery mildew and the disease-resistant of 300 plants from its F_2 generation was identified and analyzed with x~2 Test Method.The nursery of DNA susceptible plant and DNA resistant plant of the F_2 generation would be established with Group Segregation and Analysis Method(BSA) and 165 pairs of primers that were uniformly distributed in 21 linkage groups of wheat were used to do the selection of the SSR primer of the nursery of DNA resistant plant and its parents.Results]The resistance of SARKA was controlled by a pair of recessive gene,which separation was 1:3 of a pair of allele genes after x~2 Test.Conclusion]The SSR marker;Xgwm 99 and Xgwm 357,in chromosome 1A was highly linked with the resistance gene to powdery mildew of SARKA,which genetic distance were 34.7 and 29.9 cM,respectively.