紫花苜蓿中多糖的提取方法研究

目的]探究提取紫花苜蓿中多糖的最佳工艺条件。方法]采用热水浸提法提取紫花苜蓿中多糖,并通过单因素试验和正交试验研究了浸提时间、浸提温度、浸提固液比、浸提次数对提取紫花苜蓿中多糖得率的影响。结果]紫花苜蓿中多糖的最佳提取工艺条件为浸提时间1 h、浸提温度95℃、固液比1∶30、浸提3次。在该最佳提取条件下,多糖的得率可达6.1%。结论]为紫花苜蓿多糖的研究和生产提供了理论依据。

Study on the Extraction Method of Polysaccharide from Medicago Sativa

Objective ] The aim was to study the optimal extraction conditions of polysaccharide from Medicago sativa. Method ] The polysac- charide was extracted from Medicago sativa by using hot water extraction method. Effects of four factors like extraction time, extraction tempera- ture, solid-liquid ratio and extraction times on the extracting rate of polysaccharide extracted from Medicago sativa were studied through single-fac- tor experiment and orthogonal experiment. Result ] The optimal extraction conditions of polysaccharide from Medicago sativa were as followed ： the extraction time was 1 h ; the extraction temperature was 95 ℃ ; the solid-liquid ratio was 1 ： 30 ; and the extracting time was three times. Under the above conditions, the extracting rate of polysaccharide was up to 6.1%. Conclusion ] The research results provides theoretical basis for the study and production of polysaccharide from Medicago sativa.