乌贼内脏酶解多肽的制备和抗氧化活性研究

目的]探讨乌贼内脏酶解多肽的制备和抗氧化活性。方法]以乌贼内脏为原料,用胰蛋白酶对其进行水解,采用单因素和正交试验方法研究酶解温度、酶解时间、加酶量和pH对水解度和抗氧化性的影响;通过检测羟自由基清除率、DPPH清除率和还原力评价不同分子量水解物多肽的抗氧化性。结果]当酶解温度为35℃,时间为5 h,加酶量为5 000 U/g,pH为7.5时,水解度最大;当酶解温度为40℃、时间为7 h、加酶量为4 500 U/g,pH为8.0时,水解物对羟自由基清除率最大。超滤后不同分子量多肽对羟自由基的清除率为3～5 kD3 kD以下5～10 kD;DPPH清除率:3～5 kD3 kD以下5～10 kD;还原力:3 kD以下3～5 kD5～10 kD。结论]乌贼内脏酶解多肽具有较好的抗氧化活性,不同分子量多肽的活性不同。

Study on Preparation and Antioxidant Activity of Enzymatic Hydrolysis Peptide from Squid Organ

Objective] To discuss the preparation and antioxidant activity of enzymatic hydrolysis peptide of squid organ. Method ] Peptide was extracted from squid organ by Trypsin, the effects of enzymolysis temperature, time, enzyme dose and pH on degree of hydrolysis and antiox- idant activity were studied through single factor and orthogonal experiment. Different molecular weight peptide oxidation resistance was evaluated by testing the scavenging activity of hydroxyl free radicals, and i＇educing power. Result] Degree of hydrolysis reached to maximum under the conditions of enzymolysis temperature 35 ℃, enzymolysis time 5 h, total enzyme dose 5 000 U/g and pH 7.5. The scavenging activity of hydroxyl free radicals reached to maximum under the conditions of enzymolysis temperature 40 ℃, enzymolysis time 7 h, total enzyme dose 4 500 U/g, and pH 8.0. The peptides was fractionated according to molecular weights （MW） using three different molecular weight cut-off membranes （ 10, 5 and 3 kDa） and were separated to three fractions, 3 -5 kD with the highest hydroxyl free radicals scavenging activity and 〈 3 kD with the high- est reducing power. Conclusion] Hydrolysis peptide of squid organ has a good antioxidant activity and differed in molecular weight.