石榴皮中有效成分的提取及增白效果的研究

目的]对石榴皮中有效成分进行提取,并对提取物的增白效果进行研究。方法]以石榴皮为原料,通过单因素试验确定石榴皮中有效成分提取的最佳工艺,同时检测其提取物对酪氨酸酶的抑制作用,探讨石榴皮中有效成分的增白效果。结果]超声波提取石榴皮中有效成分的最佳条件是:1∶20料液比,提取温度60℃,提取液60%乙醇溶液,超声波处理强度7,处理时间40 min,在此条件下提取得到的石榴皮提取物对酪氨酸酶具有较强的抑制作用。普通溶剂提取法所得提取物对酪氨酸酶的抑制率为60%左右,而在超声波辅助提取最佳条件下得到的提取物对酪氨酸酶活性抑制率达90%;同时提取物对酪氨酸酶的抑制作用随着提取物的浓度增大而增高,最高可达100%。结论]为将石榴皮开发为美白化妆品的天然绿色原料提供了一定的理论依据。

Extraction of Effective Ingredient from Pomegranate Peels and Its Whitening Effect

Objective] This study aimed to extract the effective ingredient of pomegranate peels and explore its whitening effect.Method] Pomegranate peels were used as materials for single factor experiment to determine the optimal technique for effective ingredient extraction from pomegranate peels and explore the inhibition of the extracts on tyrosinase and whitening effect of the effective ingredient of pomegranate peels.Result] The optimal conditions for ultrasound-assisted extraction of effective ingredient in pomegranate peels were: liquid-to-solid ratio of 1∶ 20 with 60% ethanol,extraction temperature of 60 ℃,ultrasonic intensity of 7 and processing time of 40 min,which led to relatively strong inhibition effect of pomegranate peel extracts on tyrosinase.The inhibition rate of extracts obtained with common solvent reflux extraction method was only around 60%,while the inhibition rate of extracts obtained with ultrasound-assisted extraction method on tyrosinase activity had achieved around 90%,showing a very significant difference,which indicated that during the process of extraction of effective ingredient from pomegranate peels,appropriate ultrasound-assistance was advantageous to the extraction of active ingredients;in addition,the inhibition effect of extracts on tyrosinase had increased with the increasing concentration of extracts,and the inhibition rate could achieve 100%.Conclusion] This study provided a certain theoretical basis for the utilization of pomegranate peels as natural green materials to develop whitening cosmetics.