细枝木麻黄组织培养和转基因研究

目的]对细枝木麻黄进行组织培养和转基因研究。方法]以耐寒性细枝木麻黄为试验材料,探讨了转化受体选择压力、菌液浓度、共培养时间在愈伤组织诱导及不定芽分化方面对农杆菌介导转基因转化效率的影响。结果]对细枝木麻黄不定芽诱导分化适宜的激素组合为DCR+6-BA 5.0 mg/L+NAA 0.5 mg/L;转基因抗生素选择压力为潮霉素,共培养时间3 d;以初步建立的转基因体系利用农杆菌介导法获得94株转基因木麻黄,通过PCR检测,其中61株为PCR阳性植株。结论]该研究为细枝木麻黄的组织培养和转基因研究奠定了基础。

A Preliminary Study on Tissue Culture and Transgene of Casuarina equisetifolia L

Objective] The paper aimed to study the tissue culture and transgene of C.equisetifolia.Method] C.equisetifolia were used as experimental materials to explore the effects of three conditions including callus induction,adventitious bud differentiation and Agrobacterium-mediated transformation on transformation rate of C.equisetifolia.Result] The appropriate plant growth regulator combination on induction and differentiation of C.equisetifolia adventitious buds was DCR+5.0 mg/L of 6-BA+0.5 mg/L of NAA;hygromycin was selected for the selective pressure and co-culture time was 3 d;94 stains of transgenic C.equisetifolia were obtained with the initially-established transgene system via Agrobacterium-mediated method,and 61 stains were PCR-positive plants according to the results of PCR detection.Conclusion] The study had laid the foundation for tissue culture and transgene research of C.equisetifolia.