猪MSTN基因生物信息学分析

目的]对猪MSTN基因进行生物信息学分析。方法]以从GenBank中检索到的猪、大鼠、小鼠、狗、绵羊、山羊、牛、黑猩猩、人、马、鸡和斑马鱼的MSTN基因CDS序列为材料,将该12个物种的MSTN蛋白序列调入DNAStar软件的Megalign程序,进行系统进化分析;并对猪MSTN基因的基本信息、内切酶图谱、编码蛋白二级结构、信号肽、跨膜结构以及蛋白质亚细胞定位进行分析。结果]猪MSTN基因与大鼠、小鼠、狗、绵羊、山羊、牛、黑猩猩、人、马亲缘关系很近;该基因包含多个酶切位点;其编码的蛋白是一个疏水性不稳定蛋白,分子量为42 791.3 u,等电点为6.98,包含375个氨基酸残基;蛋白二级结构上,含有20.53%的α-螺旋(Helix)、4%β-转角(Turn)、53.07%无规则卷曲(Coil)、22.4%伸展条(extenden strand)和1个跨膜结构区域;该蛋白定位于细胞外,作为信号肽的可能性很大。结论]该研究为猪MSTN基因的进一步分析研究提供了参考依据。

Bioinformatics Analysis on MSTN Gene of Swine

Object] The aim was to make bioinformatics analysis on MSTN gene in swine.Method] In the research,coding sequences(CDS) of MSTN gene in swine,rat,mouse,dog,sheep,goat,cattle,chimpanzee,human,horse,chicken and zebra fish were loaded to Megalign of DNAstar for analysis of phyletic evolution.In addition,we also made analysis on basic information,restriction map,secondary structure of coding protein,signal peptide,transmembrane domain and protein subcellular localization.Result] Closely related to rat,mouse,dog,sheep,goat,cattle,chimpanzee,human and horse,MSTN genes in swine included many enzyme cutting sites and the encoding protein was unstable in hydrophobicity.The molecular weight was 42 791.3 u,isoelectric point was 6.98,and the gene included 375 amino acid residues.In addition,the secondary structure of protein contained 20.53% of α-Helix,4% of β-Turn,53.07% of Coil,22.4% of extenden strand and one transmembrane domain.The extracellular protein was probably being signal peptide.Conclusion] The research provides references for further study on MSTN genes of swine.