小菜蛾化学感受蛋白突变体的构建 |
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引用本文: | 刘晶晶,胡美英,任珍珍,李亚楠.小菜蛾化学感受蛋白突变体的构建[J].安徽农业科学,2012,40(3):1343-1345. |
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作者姓名: | 刘晶晶 胡美英 任珍珍 李亚楠 |
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作者单位: | 华南农业大学昆虫毒理研究实验室,广东广州,510642;华南农业大学昆虫毒理研究实验室,广东广州,510642;华南农业大学昆虫毒理研究实验室,广东广州,510642;华南农业大学昆虫毒理研究实验室,广东广州,510642 |
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基金项目: | 广东省自然科学基金,广东省科技项目 |
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摘 要: | 目的]研究半胱氨酸(Cys58)对小菜蛾(Plutella xylostella)化学感受蛋白1(PlxyCSP1)与农药化合物结合特性的影响。方法]利用重叠延伸PCR法,将PlxyCSP1中Cys58突变成色氨酸(Trp58),获得突变体PlxyCSP1-M2。构建表达载体,表达突变蛋白,并进行Western Blot检测。结果]构建了突变基因的原核蛋白表达载体pET32a-PlxyCSP1-M2,并表达了大小为35 kDa的融合蛋白。结论]利用重叠延伸PCR法成功在原核系统中表达了PlxyCSP1突变蛋白。
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关 键 词: | 小菜蛾 化学感受蛋白1 原核表达 |
A Mutant of PlxyCSP1 Protein in Plutella xylostella |
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Institution: | LIU Jing-jing et al(Laboratory of Insect Toxicology,South China Agricultural University,Guangzhou,Guangdong 510642) |
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Abstract: | Objective] This study aimed to explore the effects of cysteine(Cys58)on the characteristics of PlxyCSP1 in P.xyostella combined with pesticide compounds.Method] Cys58 of PlxyCSP1 was mutated into Trp58 by using overlap extension PCR method,and PlxyCSP1-M2 mutant was obtained.Expression vector was constructed and the protein was detected by western blot.Result] Expression vector pET32a-PlxyCSP1-M2 was constructed to express the 35 kDa weight protein.Conclusion] PlxyCSP1 mutant protein was expressed successfully in prokaryotic expression system. |
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Keywords: | P xylostella PlxyCSP1 Prokaryotic expression system |
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