| 禽类PRL基因的cDNA克隆及生物信息学分析 |
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| 引用本文： | 淮亚红,孙俊丽,何迟垚.禽类PRL基因的cDNA克隆及生物信息学分析[J].安徽农业科学,2012,40(3):1315-1317. |
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| 作者姓名： | 淮亚红 孙俊丽 何迟垚 |
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| 作者单位： | 中山火炬职业技术学院,广东中山,528436;广西壮族自治区畜牧研究所,广西南宁,530001;广东春盛生物科技发展有限公司,广东广州,510870 |
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| 基金项目： | 中山市农业科技攻关项目 |
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| 摘 要： | 目的]通过对PRL基因(催乳素基因)的克隆及生物信息学分析,研究该基因编码的蛋白对禽类就巢性的作用。方法]利用原鸡PRL基因mRNA序列(NM-205466)与蓝孔雀表达序列标签(EST)数据库进行Blast检索,以及序列拼接和逆转录-聚合酶链式反应(RT-PCR),获得了蓝孔雀PRL基因的部分cDNA序列,并对该序列进行了生物信息学分析。结果]获得的cDNA序列片段长度为927bp,包括完整的开放阅读框690 bp,编码229个氨基酸。系统发育树的构建发现蓝孔雀与原鸡亲缘关系比较近,与鹑次之,与火鸡亲缘关系最远。结论]该研究结果为研究PRL蛋白的生物学功能奠定了基础。
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| 关 键 词： | PRL基因 组织表达 生物信息学 |
Gene Expression and Bioinformatics Analysis of Poultry PRL Gene |
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| Institution: | HUAI Ya-hong et al (Zhongshan Torch Polytechnic,Zhongshan,Guangdong 528436) |
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| Abstract: | Objective] The aim was to study protein functiorr encoded by PRL gene(Prolactin gene) in the poultry broodiness by cloning and bioinformatics analysis.Method] Based on blast searches with the mRNA sequence of Gallus PRL and the Pavo-expressed sequence tags databases,a cDNA fragment of PRL gene was obtained by RT-PCR.Bioinformatics analysis on it was performed.Result] The obtained cDNA fragment was 927 bp,included 690 bp integrated ORF which encoded 229 amino acids.Phylogenetic tree analysis showed that Gallus is closer to Pavo than Coturnix in genetic relationship,turkey is farthest.Conclusion] The research has laid the foundation for the PRL protein biological function. |
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| Keywords: | PRL gene Tissue Expression Bioinformatics |
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