茶多酚和茶多糖清除羟基自由基的效果

目的]研究茶多酚、茶多糖对羟基自由基(.OH)的清除效果,为茶叶的药理作用提供理论依据。方法]采用Fenton法产生.OH使溴甲酚紫褪色的反应,在反应中加入一定剂量的茶多酚和茶多糖,测定它们清除.OH的效果,并同硫脲的作用进行比较。结果]1.0mg/ml茶多酚在剂量0.4 ml时对.OH清除率最大,达到76.04%。1.0 mg/ml茶多糖在剂量0.5 ml时对.OH清除率最大,达到80.97%。0.1～0.3 ml 1.0 mg/ml茶多糖对.OH的清除率显著高于同剂量和浓度下的茶多酚(P<0.05或0.01),但剂量达到0.4 ml后,两者的作用无显著差异(P>0.05)。0.1 mg/ml硫脲在剂量0.6 ml时对.OH清除率最大,达到64.28%。结论]茶多酚、茶多糖在体外对.OH有显著的清除作用,且在一定剂量范围内清除率与剂量呈线性关系,但茶多酚和茶多糖对.OH的清除率不及硫脲。

The Scavenging Effects of Tea Polyphenols and Tea Polysaccharide on·OH

Objective] The aim was to study the scavenging effects of tea polyphenols and tea polysaccharide on ·OH,to provide theoretical basis for pharmacological function on tea.Method] Producing ·OH with Fenton reaction and bromcresol purple was faded,a dose of tea polyphenols and tea polysaccharide were added to the reaction,and the clearance rate of ·OH was determined and compared with thiourea.Result] 0.4 ml dose of 1.0 mg/ml tea polyphenls had maximum clearance and reached 76.04%,the 0.5 ml dose of 1.0 mg/ml tea polysaccharide had maximum clearance and reached 80.97%.The clearance of 0.1-0.3 mg/ml tea polysaccharide on ·OH was higher than tea polyphenls significantly under the same dose and volume(P<0.05 or 0.01),but after the dose reached to 0.4 ml,the effects of two had not significant difference(P>0.05).The clearance of 0.6 ml dose of 0.1 mg/ml thiourea on ·OH was maximum and reached 64.28%.Conclusion] The tea polyphenls and tea polysaccharide had marked function of clearing ·OH in vitro,and in certain range of dosage,there was linear relationship between the clearance rate and dose.But clearance of thiourea was higher than tea polyphenls and tea-polysaccharide significantly.