烟台威海沿海菲律宾蛤仔线粒体遗传多样性分析

目的]了解我国沿海野生菲律宾蛤仔群体遗传多样性和遗传分化情况。方法]采用PCR技术对烟台威海2个群体的线粒体COⅠ基因片段进行扩增和序列分析。结果]50个样本均得到684 bp的COⅠ基因片段序列,共定义了42个单倍型,检测到62个变异位点,2个群体单倍型多样性都较高,而核苷酸多样性相对偏低。用最大似然法(Maximum Likelihood,ML)构建的单倍型系统进化树显示2个地理种群的样本呈现散在分布,无明显的种群分化。结论]反映了海洋动物的基因交流较容易,不同海域隔离较弱。

Analysis on Genetic Diversity of Mitochondria from Venerupis philippinarum in the Coasts of Yantai and Weihai

Objective] The aim was to understand genetic diversity and genetic differentiation of wild Venerupis philippinarum population in the coast of China.Method] Based on PCR technology,COⅠ gene fragments of the mitochondria from two populations of Yantai and Weihai were amplified and analyzed.Result] Fifty samples all obtained COⅠ gene fragment of 684 bp.A total of 42 haplotypes were defined,and 62 variation sites were detected.The haplotypes diversity of two populations was both high,while the nucleotide diversity was low.The phylogenetic tree of haplotypes established by maximum likelihood(ML) method showed that the samples of two geographic populations presented diffused distribution and had no obvious population differentiation.Conclusion] The result indicated that the gene exchange of marine animal was easy,and the separation among different seas was weak.