| 新疆泥火山土壤宏基因组DNA的提取方法研究 |
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| 引用本文： | 李建辉,路盼盼,张亚平.新疆泥火山土壤宏基因组DNA的提取方法研究[J].安徽农业科学,2010,38(23):12416-12417. |
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| 作者姓名： | 李建辉 路盼盼 张亚平 |
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| 作者单位： | 石河子大学生命科学学院,新疆石河子,832003;石河子大学生命科学学院,新疆石河子,832003;石河子大学生命科学学院,新疆石河子,832003 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 目的]寻找一种有效的泥火山土壤宏基因组DNA的提取方法。方法]分别采用CTAB法、SDS加酶法、实验室改进法、试剂盒法提取泥火山土壤宏基因组,比较4种方法的提取效果。结果]试剂盒法的提取效率最高、纯度最好;实验室改进法次之;SDS加酶法提取量最多。实验室改进法获得的DNA稀释10倍可直接用于PCR扩增。结论]从经济和DNA质量的角度考虑,实验室改进法可作为新疆泥火山土壤宏基因组DNA提取最有效的方法。
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| 关 键 词： | 泥火山 宏基因组DNA 新疆 |
Study on Extraction Method of Metagenomic DNA from Mud Volcano in Xinjiang |
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| Institution: | LI Jian-hui et al (College of Life Science,Shihezi University,Shihezi,Xinjiang 832003) |
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| Abstract: | Objective] The aim was to seek an effective extraction method of metagenomic DNA from Mud Volcano. Method]The metagenomic DNA from Mud Volcano was extracted by CTAB extraction method,SDS-enzyme method,improved method,reagent sets method,respectively. The extraction of four kinds of methods were compared. Result] The extracted rate and purity in reagent sets method were the highest,next was improved method,the extracted quantity in SDS-enzyme method was maximum. Ten times diluted DNA which extracted by the improved method could be used for PCR. Conclusion]Considering economy and DNA quality,the improved method could be used as an effective extraction method of metagenomic DNA from Mud Volcano. |
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| Keywords: | Mud volcano Metagenomic DNA Xinjiang |
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