| 细叶远志愈伤组织的诱导 |
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| 引用本文： | 曲伟红,赵建国,刘可越.细叶远志愈伤组织的诱导[J].安徽农业科学,2011,39(26):15975-15976. |
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| 作者姓名： | 曲伟红 赵建国 刘可越 |
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| 作者单位： | 九江学院基础医学院药学教研室,江西九江,332000 |
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| 基金项目： | 江西省科技厅自然基金项目(2007GQY0140); 江西省卫生厅中医药科研基金项目(2007B11) |
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| 摘 要： | 目的]探讨影响细叶远志（Polygala tenuifolia Willd.）愈伤组织诱导的因素,建立细叶远志愈伤组织培养方法。方法]采用组织培养方法,比较不同外植体、pH、温度、光照、激素种类及配比对细叶远志愈伤组织诱导的影响。结果]在培养基MS上,子叶的诱导率最高,为88%;叶片和茎诱导生成的愈伤组织较少;不同pH对愈伤组织生长质量的影响差异不明显;愈伤组织诱导最适温度为28℃;66%的光照培养诱导率高于33%的光照培养;1.0 mg/L6-BA＋0.1 mg/L NAA的激素配比的诱导率最高。结论]细叶远志愈伤组织的适宜培养基为MS＋1.0 mg/L6-BA＋0.1 mg/LNAA＋30 g/L蔗糖＋5 g/L琼脂（pH6.8）,培养温度为28℃,66%的光照培养18 h和黑暗培养6 h交替进行。
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| 关 键 词： | 细叶远志 愈伤组织 诱导 |
Callus Induction of Polygala tenuifolia Willd |
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| QU Wei-hong et al.Callus Induction of Polygala tenuifolia Willd[J].Journal of Anhui Agricultural Sciences,2011,39(26):15975-15976. |
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| Authors: | QU Wei-hong |
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| Institution: | QU Wei-hong et al(Division of Social Science and Public Health,School of Basic Medical Sciences,Jiujiang University,Jiujiang,Jiangxi 332000) |
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| Abstract: | Objective] To study the factors influencing the callus induction of Polygala tenuifolia Willd.,so as to establish the callus culture method.Method] The tissue culture method was adopted to compare the effects of different explants,pH,temperature,sunlight,hormone types and combinations on callus induction of Polygala tenuifolia Willd..Result] The induction rate of cotyledon on Ms was the highest of 88%;the callus induced by leaves and stems were few;the effect of different pH on the callus growth was not ... |
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| Keywords: | Polygala tenuifolia Willd Callus Induction |
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