高效液相色谱-四通道紫外可见检测法测定五加皮中原儿茶酸含量

目的]建立五加皮中原儿茶酸的含量测定方法。方法]采用高效液相色谱-四通道紫外可见检测法,以ODSC18（柱长250 mm,内径4.6 mm,涂布厚度5μm）为色谱柱,流动相为甲醇∶磷酸二氢钠缓冲溶液（浓度0.1 mol/L）=15∶85,pH=2.5,测定五加皮中原儿茶酸的含量。四通道紫外检测的定性检测波长分别为245、260、275、290 nm;定量检测波长为260 nm。结果]原儿茶酸在0.5~6.0 mg/L范围内线性关系良好（r=0.999 9）,平均回收率为99.87%,RSD为2.11%。结论]该方法测定结果可靠,稳定易行,重复性好,可用于五加皮中原儿茶酸的含量测定。

Determination of Protocatechuic Acid in CORTEX ACANTHOPANACIS Using High Proficiency Liquid Chromatography Coupled with Four-channel UV-visible Detector

Objective]To established a quantitative method of determination of protocatechuic acid in cortex acanthopanacis radicis.Method] High proficiency liquid chromatography(HPLC) coupled with four-channel UV-visible detector was used,C18 chromatographic column,mobile phase was the buffer solution of methanol and sodium dihydrogen phosphate(0.1 mol/L),the volume ratio of methanol and sodium dihydrogen phosphate was 15∶85,pH=2.5.Qualitative evaluation was done by retention time and four-channel UV spectrum at the same time,and the detection wavelength were 245 nm,260 nm,275 nm,290 nm.Selected the best sensitive wave of 260 nm to make quantitative evaluation.Result:The linearity of protocatechuic acid was in the range of 0.5-6.0 ml/L(r=0.999 9),the average recovery was 99.87%,the RSD was 2.11%.Conclusion]The method is reliable,and can be used for quality control of cortex acanthopanacis radicis.