中药秦艽基原植物的DNA分子鉴定

目的]分析秦艽基原植物间不同DNA序列的差异,为秦艽药材DNA条形码的筛选和基原鉴定提供分子证据。方法]采用PCR扩增纯化后直接测序的方法,测定大叶秦艽（G.macrophylla Pall.）、麻花艽（G.straminea Maxim.）、粗茎秦艽（G.crassicaulis Duthie exBurk.）、小秦艽（G.dahurica Fisch）、黄管秦艽（G.officinalis H.Smith）5种植物的核糖体DNA ITS、叶绿体DNApsbA-trnH核苷酸序列,并作序列同源性分析。结果]cpDNApsbA-trnH序列长度变异范围为316~318 bp,有7种不同的单倍型,单倍型间有7个变异位点,序列的GC含量为21.2%;最大简约树的聚类结果与单倍型反映的结果一致。nrDNAITS序列长度变异范围为624~625 bp,有5种不同的单倍型,单倍型间有12个变异位点,序列的GC含量为59.3%;最大简约树的聚类结果表明,小秦艽与麻花艽聚为一支,大叶秦艽与黄管秦艽聚为一支,粗茎秦艽位于聚类图的最基部。结论]nrDNAITS序列较适合作秦艽基原植物的DNA分子鉴定。

DNA Molecular Identification of Botanical Origin in Chinese Herb Qingjiao

Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evaluation.Methods] The cpDNA psbA-trnH and nrDNA ITS sequences of five botanical origin plants from Chinese herb Qingjiao,including Gentiana macrophylla Pall.,G.straminea Maxim.,G.crassicaulis Duthie ex Burk.,G.dahurica Fisch and G.officinalis H.Smith,were amplified with PCR,and then sequenced by d...