| 秋水仙素诱导山东丹参丛生芽多倍体的研究及鉴定 |
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| 引用本文： | 房翠萍,单成钢,王维婷,陈庆亮,倪大鹏,王志芬.秋水仙素诱导山东丹参丛生芽多倍体的研究及鉴定[J].安徽农业科学,2011,39(24):14629-14631. |
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| 作者姓名： | 房翠萍 单成钢 王维婷 陈庆亮 倪大鹏 王志芬 |
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| 作者单位： | 山东省农业科学院农产品研究所,山东济南250100;安徽农业大学生命科学院,安徽合肥230036;山东省农业科学院农产品研究所,山东济南,250100 |
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| 基金项目： | 农业部行业科技专项(200803034); 山东省博士后创新项目(200903017) |
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| 摘 要： | 目的]探究秋水仙素对山东丹参丛生芽诱导的可行性。方法]采用秋水仙素处理方法诱导山东丹参丛生芽。结果]添加12mg/L秋水仙素时,多倍体诱导率最高（36.6%）,存活率只有16.7%;添加8 mg/L秋水仙素时,多倍体诱导率为15.9%,成活率最高（40.0%）。多倍体山东丹参的植株粗壮,叶片大而厚,颜色深,叶片下表皮上的气孔密度小,长度长。结论]秋水仙素能有效诱导丛生芽,并获得了丹参多倍体育种材料,为缩短育种进程奠定了基础。
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| 关 键 词： | 秋水仙素 山东丹参 丛生芽 多倍体 气孔鉴定 |
Study on the Identification of Polyploidy of Salvia miltiorrhiza Multiple Shoots Induced by Colchicine in Shandong |
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| FANG Cui-ping et al.Study on the Identification of Polyploidy of Salvia miltiorrhiza Multiple Shoots Induced by Colchicine in Shandong[J].Journal of Anhui Agricultural Sciences,2011,39(24):14629-14631. |
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| Authors: | FANG Cui-ping |
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| Institution: | FANG Cui-ping et al(Institute of Agricultural products,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100) |
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| Abstract: | Objective] The aim was to explore the feasibility of using colchicine to induce the multiple shoots of Salvia miltiorrhiza in Shandong.Method] The multiple shoots of Salvia miltiorrhiza in Shandong were induced by colchicine.Result] When the concentration of colchicine supplemented in solid medium was 12 mg/L,the induction rate of polyploidy was the highest(36.6%),but its survival rate was only 16.7%.Adding 8 mg/L colchicine,the induction rate of polyploidy was 15.9%,while its survival rate was up to 40.... |
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| Keywords: | Colchicine Salvia miltiorrhiza in Shandong Multiple shoots Polyploidy Stomata identification |
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