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玫瑰花对β-淀粉样蛋白诱导PC12神经细胞毒性的抑制作用
引用本文:杨庆雄,王聪聪,张万全,余天华,孙黔云.玫瑰花对β-淀粉样蛋白诱导PC12神经细胞毒性的抑制作用[J].安徽农业科学,2011,39(27):16548-16550.
作者姓名:杨庆雄  王聪聪  张万全  余天华  孙黔云
作者单位:1. 贵州师范大学化学与材料科学学院,贵州贵阳,550001
2. 贵州师范大学生命科学学院,贵州贵阳,550001
3. 贵州省中国科学院天然产物化学重点实验室,贵州贵阳,550002
基金项目:国家自然科学基金项目(30860038); 贵州省科技厅科技基金项目(黔科合J字[2009]2090号)
摘    要:目的]研究玫瑰花提取物对β-淀粉样蛋白(Aβ25-35)诱导的损伤PC12细胞的保护作用。方法]PC12细胞加入Aβ25-35共同孵育后,采用四甲基偶氮唑盐(MTT)法检测PC12细胞活力,比色法检测细胞内丙二醛(MDA)含量,酶联免疫法检测细胞内Nf-κb水平。结果]玫瑰花提取物对PC12无显著细胞毒性,可以显著抑制Aβ25-35诱导的PC12细胞毒性。与模型组相比,添加玫瑰花乙酸乙酯萃取物的样品组能降低PC12细胞内MDA浓度和Nf-κb表达。结论]玫瑰花提取物对β-淀粉样蛋白致PC12细胞损伤具有一定的抑制作用,其作用机理可能与降低Aβ25-35所致的PC12细胞内的氧化应激有关。

关 键 词:玫瑰花(FLOSROSAERUGOSAE)  PC12细胞  β-淀粉样蛋白  氧化应激

Inhibition Effects of FLOS ROSAE RUGOSAE Extracts on the Cytotoxity of Aβ25-35-induced PC12 Cell
YANG Qing-xiong et al.Inhibition Effects of FLOS ROSAE RUGOSAE Extracts on the Cytotoxity of Aβ25-35-induced PC12 Cell[J].Journal of Anhui Agricultural Sciences,2011,39(27):16548-16550.
Authors:YANG Qing-xiong
Institution:YANG Qing-xiong et al(College of Chemistry and Material Science,Guizhou Normal University,Guiyang,Guizhou 550001)
Abstract:Objective] To study the neuroprotection effect of FLOS ROSAE RUGOSAE on Aβ25-35-induced PC12 cells.Method] PC12 cell was co-incubated with Aβ25-35,and then the cell viability was determined by MTT method,content of MDA was determined by colorimetry,and the expression level of Nf-κb was evaluated by ELISIA.Result] The extract of FLOS ROSAE RUGOSAE did not show apparent cytotoxity towards PC12 cell line,instead,it inhibited the cytotoxity induced by the Aβ25-35.Compared with the model group,the content of MDA and expression of Nf-κb in PC12 cell decreased after being treated with the extract of FLOS ROSAE RUGOSAE.Conclusion] The extracts of FLOS ROSAE RUGOSAE showed neuroprotection effect on the cytotoxity of PC12 induced by Aβ25-35,and the mechanism of the neuroprotection may be related to the decreasing content of oxidative stress in the PC12 cell.
Keywords:FLOS ROSAE RUGOSAE  PC12 cell  Aβ25-35  Oxidative stress
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