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| 磷酸盐缓冲液的浓度和pH对革胡子鲶肠道抗菌蛋白/肽分级盐析的影响 | |
| 引用本文： | 王存,王晓梅,季延滨,徐敏,戴伟,潘宝平.磷酸盐缓冲液的浓度和pH对革胡子鲶肠道抗菌蛋白/肽分级盐析的影响[J].安徽农业科学,2011,39(28):17344-17346,17394. |
| 作者姓名： | 王存 王晓梅 季延滨 徐敏 戴伟 潘宝平 |
| 作者单位： | 1. 天津师范大学生命科学学院,天津300387;天津市水产生态及养殖重点实验室,天津农学院水产科学系,天津300384 2. 天津市水产生态及养殖重点实验室,天津农学院水产科学系,天津300384 3. 天津师范大学生命科学学院,天津,300387 |
| 基金项目： | 天津市应用基础及前沿技术研究计划重点研究项目(08JCZDJC19000) |
| 摘 要： | 目的]探讨磷酸盐缓冲液的pH和浓度在分级盐析时,对革胡子鲶肠道各组分蛋白/肽的影响。方法]以不同浓度和pH的磷酸盐缓冲液作为组织匀浆缓冲液,在硫酸铵饱和度为20%、40%、60%、80%和100%的条件下,对革胡子鲶肠道抗菌蛋白/肽进行分级盐析,并对盐析产物进行称重、电泳检测和抑菌活性分析。结果]磷酸盐缓冲液的pH为6.0或7.4时,其浓度对盐析产物产量、蛋白沉淀和抑菌效果无显著影响。而pH为8.0、浓度为0.05 mol/L时,硫酸铵饱和度为60%和100%的粗蛋白/肽的产量显著高于浓度为0.02 mol/L的磷酸盐缓冲液粗蛋白含量;大分子蛋白能更好地在硫酸铵饱和度为20%和40%时沉淀下来,而在饱和度为60%、80%和100%能获得分子量相对较小的抗菌蛋白/肽。磷酸盐缓冲液的pH和浓度对抑菌效果无显著影响。结论]pH8.0,浓度为0.05 mol/L的磷酸盐缓冲液更适于革胡子鲶肠道抗菌蛋白/肽的提取。 |
| 关 键 词： | 革胡子鲶 肠道 抗菌肽 磷酸盐缓冲液 |
Effect of Concentration and pH Value of Phosphate Buffer on Gradient Ammonium Sulphate Fractionation of Antibacterial Protein/peptide from Intestine of African Catfish(Clarias gariepinus) |
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| WANG Cun et al.Effect of Concentration and pH Value of Phosphate Buffer on Gradient Ammonium Sulphate Fractionation of Antibacterial Protein/peptide from Intestine of African Catfish(Clarias gariepinus)[J].Journal of Anhui Agricultural Sciences,2011,39(28):17344-17346,17394. | |
| Authors: | WANG Cun |
| Institution: | WANG Cun et al(College of Life Science,Tianjin Normal University,Tianjin 300387) |
| Abstract: | Objective] The effects of the concentration and pH value of phosphate buffer on the proteins/peptides in each fraction of African catfish in testine were studied.Method] The antibacterial proteins/peptides from intestine of African catfish were fractionation by a graded ammonium sulfate(AS) precipitation technique and the fractions were obtained at 20%,40%,60%,80% and 100 % saturation with ammonium sulfate.Each fraction was weight and detected by SDS-PAGE and its antibacterial activity was analyzed.Resul... |
| Keywords: | African catfish Intestine Antibacterial protein/peptide Phosphate buffer |
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