酵母菌与乳酸菌混合培养条件研究

目的]研究酵母菌与乳酸菌混合培养的条件。方法]对产朊假丝酵母（Candida tails）、酿酒酵母（Saccharomyces cerevisiae）、植物乳杆菌（Lactobacillus plantarum）、嗜酸乳杆菌（Lactobacillus acidophilus）等4种菌的混合培养条件进行了优化,分析了溶解氧、温度、pH值、接种比例及接种量对其菌体生长的影响。结果]在培养基的起始pH值6.5、酵母菌与乳酸菌混合种子（活菌数约2×109 CFU/ml）中酿酒酵母菌数：产朊假丝酵母菌数：植物乳杆菌数：嗜酸乳杆菌数约为1∶2∶3∶3、酵母菌与乳酸菌混合种子的接种量为0.2%、前期120r/min震荡培养24 h,后期静置培养24 h,培养温度为28℃恒温24 h,32℃恒温24 h条件下,培养液中的总活菌数可达到6.50×108CFU/ml。结论]该培养基增殖效果好,适合应用于大规模生产混合发酵酵母菌与乳酸菌的发酵剂。

Study on Mixed culture Conditions of Yeast and Lactic Acid Bacteria

Objective]The aim was to research the mixed culture conditions of yeast and lactic acid bacteria.Method]The mixed culture conditions of Candida tails、Saccharomyces cerevisiae、Lactobacillus plantarum、Lactobacillus acidophilus were optimized.The effects of dissolved oxygen,temperature,pH,inoculation ratio and inoculum concentration on its cell growth were analysed.Result]The results showed that the number of total viable cells could reach 6.50×108 CFU/ml under the conditions of its original pH 6.5,the number of Saccharomyces cerevisiae∶ Candida tails∶ Lactobacillus plantarum∶ Lactobacillus acidophilus was 1∶ 2∶ 3∶ 3(2×109 CFU/ml),the mixed inoculum of yeast and lactic acid bacteria was 0.2%,120 r/min for 24 h at first,then static cultre 24 h,28 ℃ for 24 h and 32 ℃ for 24 h. Conclusion]The multiplication effect of the culture medium was well and was suitable for mass production of mixed fermentation of yeast and lactic acid bacteria.