应用于景观水体异养硝化细菌的筛选鉴定及效果研究

目的]研究景观水体的生物修复技术。方法]采用SBR反应器,通过间歇曝气方式对底泥体系中好氧反硝化细菌进行了选择和富集,分离到1株异养硝化菌,并根据其生理生化性状和部分长度的16S rDNA序列确定了分离菌株的分类学和系统发育地位。结果]从底泥中分离出1株异养硝化细菌SHW1,经过生理生化鉴定和16S rDNA测序,建立了系统发育树,鉴定出细菌SHW1属于Acineto-bactersp.。采用乙酸钠-氯化铵培养基培养细菌进行硝化特性研究,经过7 d好氧培养,NH4＋-N最终去除率为52.13%,并且具有产生NO2--N的硝化性能。结论]筛选出的异养硝化细菌SHW1在贫营养条件下对NH4＋-N有较高的去除率,可以应用于景观水体脱氮。

Research on the Screening and Identification of Heterotrophic Nitrification Bacteria Applied in Landscape Water and Its Effect

Objective] The bioremediation technique for scenic water was developed.Method] One bacterial strain of heterotrophic nitrification was isolated after the aerobically denitrifying bacteria in sediment was accumulated and selected by way of intermittent aeration in SBR reactor.The taxonomy and phylogenetic position of the isolated strain was assured according to its physiological character and the partial length of 16S rDNA sequence.Result] One bacterial strain—SHW1 of heterotrophic nitrification was isolated from the sediment and the phylogenetic tree was established after its physiological and biochemical character was identified and 16S rDNA sequence was tested,indicating that the SHW1 belonged to Acinetobacter sp.The nitrification characteristics of the SHW1 cultured in the medium of sodium acetate-ammonium chloride was analyzed and the final removal rate of NH4+-N was 52.13% after 7 days' aerobic incubation and the strain was had the nitrification performance in the production of ed NO2——N.Conclusion] The bacterial strain-SHW1 had a higher removal rate of NH4+-N under the condition of less nutrient,which could be used in the de-nitrification of landscape water-body.