透明质酸对山羊卵母细胞体外成熟和孤雌胚胎发育的影响

在无血清培养体系中添加不同浓度的HA,探讨HA浓度对山羊卵母细胞体外成熟和早期胚胎发育的影响。试验结果表明,当HA浓度在1.0和1.5 mg/ml时,卵母细胞成熟率(分别为74.5%和76.3%)显著高于对照组(P<0.05),且高于其他各组,说明HA可以促进卵母细胞成熟,且浓度在1.0~1.5 mg/ml时最有利于卵母细胞成熟;当HA浓度为1.5 mg/ml时,卵裂率(85.7%)和囊胚率(44.4%)均显著高于对照组,与其他各组比较,均达最优值,说明HA可以促进早期胚胎发育,且浓度为1.5 mg/ml时,最有利于早期胚胎发育。

Effect of Hyaluronic Acid on In vitro Maturation and Early Embryos Development of Goat Iocytes

In this study,hyaluronic acid(HA) was added in the modified foundational medium,to mainly determine the effect of HA on invitro maturation of goat oocytes and development of the early embryos.The first experiment's result showed that,the groups with 1.0 and 1.5 mg/ml HA were significantly higher than the other groups in the frequency of the first polor body(PB) extrusion(74.5%,76.3%,P<0.05),this result primitively expressed that 1.0～1.5 mg/ml was the best concentration of HA in serum free medium for invitro maturation of goat oocytes.The second experiment's result showed that,the group with 1.5 mg/ml HA was significantly higher than the other groups in the frequency of cleavage rate(85.7%) and blastcyst rate(44.4%),this result expressed that 1.5 mg/ml was the best concentration of HA in serum free medium for early embryos development of goat.