青海家牦牛HIF-1α基因组织特异性表达

目的]探究青海家牦牛HIF-1α基因组织的特异性表达。方法]应用半定量反转录PCR和实时定量反转录PCR(SYBRGreen)技术对青海家牦牛HIF-1α基因的组织特异性表达进行检测。通过提取不同组织总RNA,经DNase I消化后,用随机引物进行反转录合成cDNA,采用特异性引物分别对HIF-1α和β-actin基因进行RT-PCR和Real Time RT-PCR扩增。结果]结果表明,HIF-1α基因在心、肝、脾、肺、肾、脑、肌肉、睾丸组织中均有表达,其中以睾丸和脾中HIF-1α基因表达量最高,肌肉的表达量最低。结论]该研究为进一步揭示HIF-1α在高原土著动物低氧适应过程中的分子机制有着重要的意义。

Tissue Specific Expression of HIF-1αGene in Qinghai Domestic Yak

Objective] The experiment was conducted to study the tissue specific expression of HIF-1α gene in Qinghai domestic yak.Method] Tissue specific expression of HIF-1α gene in Qinghai domestic yak was detected using the methods of Semi-quantitative RT-PCR and Real Time RT-PCR.RNA was extracted from different tissues.After treated with DNase I,the total RNA was reverse transcribed with random primer to synthesize cDNA.Then the HIF-1α gene and β-actin gene were amplified with specific primers by RT-PCR and Real Time RT-PCR.Result] Result showed that HIF-1α gene was expressed in tissues including heart,liver,spleen,lung,kidney,brain,muscle and testis.Among them,HIF-1α gene showed the highest expression amount in testis and spleen and lowest in muscle.Conclusion] This study was of significant importance for the study on the molecular mechanism of HIF-1α in plateau native animals during the process of hypoxia adaptation.