猫须草组织培养研究

目的]研究猫须草离体培养的最佳激素配比和最佳培养基。方法]以猫须草幼嫩茎段为外植体,以MS为基本培养基,比较不同浓度和不同种类的生长激素对茎段腋芽萌发、愈伤组织增殖及丛生芽生根的影响。结果]6-BA可促进腋芽萌发,分化丛生芽。随着6-BA浓度的增加,萌发腋芽的数量反而减少,当6-BA的浓度大于2.5 mg/L时,茎段便不再长出腋芽,而是在其顶端出现愈伤组织。在一定浓度范围内,随着2,4-D浓度的增加,愈伤组织的生长就越旺盛,当2,4-D浓度为2.0 mg/L时,愈伤组织增殖效果最好。NAA浓度为1.0 mg/L时诱导茎段生根效果最好,生根率达到100%。结论]适宜的诱导培养基为MS+6-BA1.0 mg/L+NAA0.1mg/L,适宜继代增殖培养基为MS+2,4-D2.0 mg/L,茎段生根的适宜培养基为MS+NAA1.0 mg/L。

Study on Tissue Culture of Clerodendranthus spicatus

Objective] The aim was to study on the best hormone matching and the best medium in vitro culture of Clerodendranthus spicatus.Method] With tender stem segment of C.spicatus as explants and MS as basic medium,the effects of different concentrations and different types of growth hormone on the germination of stem segment and axillary bud,proliferation of callus and rooting of tufty buds were compared.Result] 6-BA could promote the germination of axillary bud and differentiation of tufty buds.The germination amount of axillary bud were decreased along with increment of the concentration of 6-BA.When the concentration of 6-BA was higher than 2.5 mg/L,the axillary bud didn't grow out on the stem segment and the callus would appear on the top of stem segment.Within the range of certain concentration,along with increment of 2,4-D concentration,the callus of stem segment had more vigorous growth,and had a best proliferation effect when 2,4-D concentration was 2.0 mg/L.When NAA was at 1.0 mg/L the rooting induction of stem segment was the best(100 % of rooting).conclusion]The optimum medium for induction was MS 6-BA 1.0 mg/L NAA 0.1 mg/L,the optimum medium for subculture proliferation was MS 2,4-D 2.0 mg/L and the optimum medium for rooting of stem segment was MS NAA 1.0 mg/L.