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4种滑叶铁线莲基因组DNA提取方法比较
引用本文:胡祎晨,孙正海,王锦,李世峰,辛培尧,范萱.4种滑叶铁线莲基因组DNA提取方法比较[J].安徽农业科学,2011,39(36):22215-22216,22363.
作者姓名:胡祎晨  孙正海  王锦  李世峰  辛培尧  范萱
作者单位:1. 西南林业大学园林学院,云南昆明,650224
2. 云南省农业科学院花卉研究所,云南昆明,650205
基金项目:云南省应用基础研究项目(2010ZC089);国家林业局948项目(2008-4-11);云南省省部级重点学科、省高校重点实验室及校实验室共享平台资助项目;西南林业大学科技创新基金
摘    要:目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适DNA提取方法。方法]以滑叶铁线莲叶片为材料,比较改良CTAB法I、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取DNA纯度、浓度和提取时间等方面的不同。结果]4种方法都可提取滑叶铁线莲基因组DNA。改良CTAB法I提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短。结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持。

关 键 词:滑叶铁线莲  基因组DNA提取  改良CTAB法  改良SDS法

Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch
Institution:HU Yi-chen et al (School of Gardening,Southwest Forestry University,Kunming,Yunnan 650224)
Abstract:Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.Result] The four extraction methods could all successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.
Keywords:Clematis fasciculiflora Franch  Extraction of genomic DNA  Improved CTAB method  Improved SDS method
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