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醇提板栗壳色素对羟自由基和超氧阴离子自由基的清除作用
引用本文:何玲玲,王新,石中亮,孙伶,张英.醇提板栗壳色素对羟自由基和超氧阴离子自由基的清除作用[J].安徽农业科学,2006,34(10):2054-2054,2058.
作者姓名:何玲玲  王新  石中亮  孙伶  张英
作者单位:沈阳化工学院应用化学学院,辽宁,沈阳,110142;辽宁大学制药工程系,辽宁,沈阳,110036
摘    要:以30%乙醇溶液从板栗壳中提取得到板栗壳色素CSPⅠ,并以Fenton反应产生.OH模型、邻苯三酚自氧化反应产生O2-.模型,用紫外和可见分光光度法试验了CSPⅠ对模型中产生的.OH和O2-.的清除作用。结果表明,CSPⅠ具有良好的清除.OH作用,且清除作用与色素浓度呈正相关性,EC50为75.65 mg/L;而CSPⅠ对O2-.没有明显的清除作用。

关 键 词:板栗壳色素  抗氧化活性  羟自由基  超氧阴离子自由基  清除作用
文章编号:0517-6611(2006)10-2054-01
收稿时间:2006-03-03
修稿时间:2006-03-03

Effect of Pigment Extracted with Alcohol from Chestnut Shell on the Scavenging of Superoxide Anion and Hydroxyl Radicals
HE Ling-ling et al.Effect of Pigment Extracted with Alcohol from Chestnut Shell on the Scavenging of Superoxide Anion and Hydroxyl Radicals[J].Journal of Anhui Agricultural Sciences,2006,34(10):2054-2054,2058.
Authors:HE Ling-ling
Abstract:The pigment noted as CSP was extracted from chestnut shell with 30 % alcohol/water.The scavenging effects on hydroxyl radicals and superoxide anion radicals of the pigment from chestnut shell were studied by UV-visible spectroscopy.The hydroxyl radicals and superoxide anion radicals are produced respectively in Fenton reaction and pyrogallol autoxidation system.The results showed that CSPⅠ had the scavenging activity to hydroxyl radicals in a concentration-dependent manner.The scavenging capacity to hydroxyl radicals was represented by the concentration scavenging 50 % of all of radicals,which of CSPⅠwas 75.65 mg/L.CSPⅠ had no remarkable scavenging activity to superoxide anion radicals.
Keywords:Pigment from chestnut shell  Antioxidation activity  Hydroxyl radicals  Superoxide anion radicals  Scavenging effect
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