荷叶总黄酮的提取工艺研究

目的]为荷叶的大规模开发利用提供技术保证。方法]采用超声波辅助萃取法对荷叶总黄酮进行提取，并用分光光度法测定其含量，通过单因素及正交试验确定最佳提取工艺。结果]当乙醇浓度达到60％时，总黄酮收率最大；液料比在10：1-30：1时，黄酮的收率随液料比的增大而增加较快，当液料比大于40：1后，黄酮收率趋于恒定；当作用时间为35min时，黄酮收率最大，但此时与30min相比，仅略微增加；提取时适宜的温度应为40℃；影响荷叶总黄酮收率的主次因素顺序为乙醇浓度〉超声时间〉液料比〉超声温度。结论]荷叶总黄酮最佳提取工艺：将荷叶干粉按35：1的液料比加入浓度60％乙醇水溶液，浸泡24h，在超声波清洗槽中超声提取35min，超声温度35℃。在该条件下，荷叶总黄酮收率为13．68％。

Research on the Extraction Technology of the Total Flavonoids from Lotus Leaf

Objective] The technical support for the development and utilization of lotus leaf ata large-scale was provided.Method]The total flavonoids in lotus leaf were extracted with the ultrasound-assisted extraction method;its content was deter mined with spectrophotometric method and the optimum extraction process was studied through the single factor and orthogonal experiment.Results] When the ethanol concentration was up to 60%,the largest yield of total flavonoids could be extracted;when the ration of the s...