HPLC测定不同生长年限滇重楼中薯蓣皂苷元含量

目的]探讨滇重楼（RHIZOMA POLYGONATI）中薯蓣皂苷元的含量与生长年限的关系,为确定其最佳采收年限提供参考依据。方法]采用HPLC测定不同生长年限滇重楼中薯蓣皂苷元的含量。色谱柱为Agilent zorbax SB-C18柱（4.6mm×150.0mm,5μm）,流动相为乙腈-水（90∶10,V/V）,流速为1.0 ml/min,检测波长为203 nm,柱温为35℃。结果]不同生长年限的滇重楼中薯蓣皂苷元含量明显不同。其中,3、4年生滇重楼中薯蓣皂苷元含量较低;5、6年生滇重楼中薯蓣皂苷元含量明显升高,但6年生的含量略低于5年生;7年生滇重楼中薯蓣皂苷元含量骤升,且升幅最大。结论]不同生长年限滇重楼中薯蓣皂苷元的含量不同,在实际应用时应根据不同需要适时采收。

Determination of Diosgenin Content in RHIZOMA POLYGONATI with Different Growth Years by HPLC

Objective] To discuss the relationship between content of diosgenin in RHIZOMA POLYGONATI and its growth years,so as to provide basis for the optimal harvesting years of RHIZOMA POLYGONATI.Method] The content of diosgenin was determined by HPLC,and the HPLC conditions were :column of Agilent zorbax SB-C18column(4.6 mm×150.0 mm,5 μm),mobile phase of acetonitrile-water(V/V of 90:10),flow rate of 1.0 ml/min,detection wavelength of 203 nm and column temperature of 35 ℃.Result] Content of diosgenin in different growing years RHIZOMA POLYGONATI varied significantly.That in three years old and four years old RHIZOMA POLYGONATI was relative lower,whereas that in five,six and seven years old ones was higher and reached a peak in seven-year-old one.Conclusion] RHIZOMA POLYGONATI with different growing years differs from each other in diosgenin,and should be harvested in terms of different aims.