响应面分析法优化杜仲叶中绿原酸水提工艺

目的]优化杜仲叶中提取绿原酸水提工艺,为杜仲叶绿原酸的工业化生产提供理论依据。方法]在单因素试验的基础上,选取料液比、提取温度、提取次数、提取时间为自变量,以绿原酸的收率为响应值,采用中心组合设计,利用响应面分析法优化杜仲叶绿原酸的水提工艺。结果]杜仲叶中绿原酸水提工艺的最佳条件为：料液比1∶16,提取时间20 m in,提取次数2次,提取温度65℃。在该条件下,绿原酸的提取率达92.550%。结论]水提法与传统工艺比较,具有提取率高、能耗少、时间短和低成本等特点,为杜仲叶中绿原酸的工业化生产提供了理论依据。

Optimizaiton of Water Extraction Technology for Chlorogenic Acid from Eucommia ulmoides Oliver.Leaves by Response Surface Methodology

Objective] To optimize the water extraction conditions for chlorogenic acid from Eucommia ulmoides Oliver.leaves,so as to provide theoretical evidence for the industrialized production of Eucommia ulmoides Oliver.leaves chlorogenic acid.Method]Solid to liquid ratio,extraction temperature,extraction times and extraction duration were employed to design response surface analysis based on single factor test using yield of chlorogenic acid as response value,optimizing the extraction conditions of water extraction.Result] The optimum extraction conditions for chlorogenic acid from Eucommia ulmoides Oliver.leaves were: solid to liquid ratio of 1∶ 16,extraction duration of 20 min,extraction times of two and extraction temperature of 65 ℃.Under the optimal process,the extraction rate of chlorogenic acid from Eucommia ulmoides Oliver.leaves reached as high as 92.55%.Conclusion] In comparison with traditional extraction methods,water extraction technology was higher in extraction rate,less in energy consumption,shorter in extraction duration and lower in cost,and this case provided theoretical evidence for industrialized production of chlorogenic acid from Eucommia ulmoides Oliver.leaves.