| 亳菊茎尖愈伤组织诱导与再生植株的研究 |
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| 引用本文： | 俞年军,彭代银,吴德林,刘守金,朱月信,曹勇,王韵琴,江芹,董玲.亳菊茎尖愈伤组织诱导与再生植株的研究[J].安徽农业科学,2011,39(6):3227-3229. |
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| 作者姓名： | 俞年军 彭代银 吴德林 刘守金 朱月信 曹勇 王韵琴 江芹 董玲 |
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| 作者单位： | 安徽中医学院药学院,安徽合肥,230031;安徽中医学院现代中药安徽省重点实验室,安徽合肥,230031;安徽济人药业有限公司,安徽亳州,236800;安徽省农单科学院园艺研究所,安徽合肥,230031 |
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| 基金项目： | 2009年安徽省科技计划项目 |
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| 摘 要： | 目的]研究亳菊茎尖组织培养技术,为亳菊产业化生产提供科学依据。方法]在不同的MS培养基中,考察不同浓度的6-BA、NAA对亳菊茎尖愈伤组织、丛生芽、再生植株诱导的影响。结果]茎尖诱导愈伤组织的最佳培养基为MS＋1.00 mg/L6-BA＋0.30mg/L NAA,以MS＋0.50 mg/L6-BA＋0.10 mg/L NAA为芽的诱导培养基,芽诱导率达100%;最佳的生根培养基为1/2MS＋0.30 mg/L IBA。结论]通过亳菊茎尖愈伤组织培养技术,可以达到快速繁殖的目的。
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| 关 键 词： | 亳菊 茎尖 愈伤组织 植株再生 |
Study on Induction of Stem-tip Callus and Plant Regeneration of Dendranthemd morifolium (Ramat.)Tzvel "Boju"cv.nov |
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| Institution: | YU Nian-jun et al(College of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei,Anhui 20031) |
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| Abstract: | Objective] To study the breeding technology of Dendranthemd morifolium(Ramat.)Tzvel"Boju" cv.nov using tissue culture and provide scientific foundation for the industrialized production of Dendranthemd morifolium(Ramat.)Tzvel"Boju" cv.nov.Method]The effects of 6-BA and NAA with different concentration on induction of stem-tip callus,cluster buds and plant regeneration of Dendranthemd morifolium(Ramat.)Tzvel"Boju" cv.nov were studied.Result] The optimal culture medium for stem-tip callus and cluster buds were MS+1.00 mg/L 6-BA+ 0.30 mg/L NAA and MS+0.50 mg/L 6-BA+0.10 mg/L NAA,respectively.Whereas the optimum medium for generation of roots was 1/2MS+0.30 mg/L IBA.Conclusion] The herb Dendranthemd morifolium(Ramat.)Tzvel"Boju" cv.nov can be propagated by using stem-tip proliferation technology. |
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| Keywords: | Dendranthemd morifolium "Boju" cv nov Stem-tip Callus Plant regeneration |
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