| 香蕉品种(系)遗传多样性的SSR分析 |
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| 引用本文： | 王静毅,冀小蕊,武耀廷.香蕉品种(系)遗传多样性的SSR分析[J].安徽农业科学,2011,39(10):5706-5710. |
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| 作者姓名： | 王静毅 冀小蕊 武耀廷 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术研究所,农业部热带作物生物技术重点开放实验室,海南海口,571101;琼州学院,海南三亚,572002 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资助项目(IT-BBZD1032);海南省高校研究生创新科研课题(Hxwby2008-11) |
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| 摘 要： | 目的]从分子水平上研究香蕉种质资源的遗传多样性和亲缘关系,建立分子水平香蕉分类系统。方法]应用SSR技术从分子水平上对32个香蕉品种（系）的遗传关系进行检测。结果]39对SSR引物在32个品种（系）中扩增带数在4～20个,平均每个SSR座位可检测2.78个多态性带,引物的多态信息量（PIC）在0.45～0.91,平均0.80。依据SSR数据计算的品种间遗传距离在3.06%～43.61%,平均30.73%。结论]香蕉品种之间存在较为丰富的遗传变异。
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| 关 键 词： | 香蕉 SSR 遗传距离 聚类分析 |
SSR Analysis of the Genetic Diversity of Banana Variety(Line) |
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| Institution: | WANG Jing-yi et al(Biotechnology Research Institute of Tropical Crop,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Tropical Agriculture Crop Biotechnology of MOA,Haikou,Hanan 571101) |
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| Abstract: | Objective] The genetic diversity and relationship of the banana germplasms were analyzed at the molecular level in order to establish the classification system of bananas at molecular level.Method] The genetic relationship of 32 banana varieties(lines) was detected with the SSR technology.Results] The number of amplified band of 32 varieties(lines) based on 39 pairs of SSR primers was ranged from 4 to 20,the average 2.78 SSR polymorphic bands could be detected in each primer site and the polymorphism information content(PIC) was 0.45-0.91(average value of 0.80).The genetic distance among varieties was 3.06%-43.61%(30.73% on average) according to the calculation of SSR data.Conclusion] There was the relatively rich genetic variation among banana cultivars. |
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| Keywords: | Banana SSR Genetic distance Cluster analysis |
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