两种石斛多糖的抑菌作用研究

目的]探讨铁皮石斛多糖和金耳石斛多糖在体外对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑制作用。方法]采用滤纸片抑菌圈法研究2种石斛多糖对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑制作用。结果]铁皮石斛多糖对大肠杆菌抑制作用最强,最小抑菌浓度（MIC）为0.5%,抑菌圈直径为15.8 mm;金耳石斛多糖对枯草芽孢杆菌抑制作用最强,MIC为0.5%,抑菌圈直径为12.8 mm。结论]体外条件下,铁皮石斛多糖对大肠杆菌和枯草芽孢杆菌有抑制作用,但对金黄色葡萄球菌无抑制作用;金耳石斛多糖对大肠杆菌、金黄色葡萄球菌以及枯草芽孢杆菌均有抑制作用。

Study on the Antibacterial Effects of Two Dendrobium Polysaccharides

Objective] To research the inhibitory effects of polysaccharides from Dendrobium officinale and Dendrobium hookerianum on Staphyloccocus aureus,Escherichia coli and Bacillus subtilis in vitro.Method] The antibacterial activity of two Dendrobium polysaccharides on the above bacteria were explored by filter paper disk method.Results] Polysaccharide from Dendrobium officinale showed the most remarkable antibacterial effect on Escherichia coli,i.e.,the minimal inhibitory concentration(MIC)=0.5%,and the diameter of inhibiting zone was 15.8 mm;polysaccharide from Dendrobium hookerianum showed the most remarkable antibacterial effect on Bacillus subtilis,i.e.,MIC=0.5%,and the diameter of inhibiting zone was 12.8 mm;Conclusion] Polysaccharide from Dendrobium officinale had inhibitory effect on Escherichia coli and Bacillus subtilis,while that from Dendrobium hookerianum showed inhibitory effect on all tested bacteria.